通过实时逆转录聚合酶链反应和免疫组织化学检测缺氧诱导因子1α在鼻息肉中的表达。
Expression of hypoxia-inducible factor 1alpha in the nasal polyps by real-time RT-PCR and immunohistochemistry.
作者信息
Chien Chen-Yu, Tai Chih-Feng, Ho Kuen-Yao, Kuo Wen-Rei, Chai Chee-Yin, Hsu Ying-Che, Wang Ling-Feng
机构信息
Department of Otolaryngology, Head and Neck Surgery, Kaohsiung Medical University Hospital, Kaohsiung, Taiwan.
出版信息
Otolaryngol Head Neck Surg. 2008 Aug;139(2):206-10. doi: 10.1016/j.otohns.2008.04.022.
OBJECTIVE
Hypoxia may result in increased recruitment of inflammatory cells and release of various inflammatory cytokines. Local hypoxia within the sinus is believed to aggravate sinus inflammation. In this study, we tried to investigate the correlation of hypoxia-inducible factor, which is upregulated under hypoxic condition, and nasal polyposis.
SUBJECTS AND METHODS
Forty-four polyps from patients undergoing endoscopic sinus surgery and 38 inferior turbinate mucosa were obtained for immunohistochemical staining. Twenty-three polyps and 22 turbinate mucosa also underwent real-time reverse transcription-polymerase chain reaction for quantification of messenger RNA (mRNA). The severity of the disease was judged by the Lund-Mackay CT scoring.
RESULTS
Expression of hypoxia-inducible factor protein, but not mRNA, was significantly increased in nasal polyps compared with that in the turbinate mucosa, but it did not correlate with the disease severity.
CONCLUSION
We suggest that hypoxia may have a role in the initiation of nasal polyposis.
目的
缺氧可能导致炎症细胞募集增加以及多种炎症细胞因子释放。鼻窦内局部缺氧被认为会加重鼻窦炎症。在本研究中，我们试图探究在缺氧条件下上调的缺氧诱导因子与鼻息肉病之间的相关性。
受试者与方法
获取44例接受鼻内镜鼻窦手术患者的息肉以及38例下鼻甲黏膜用于免疫组织化学染色。23例息肉和22例鼻甲黏膜还进行了实时逆转录-聚合酶链反应以定量信使核糖核酸（mRNA）。通过Lund-Mackay CT评分判断疾病严重程度。
结果
与鼻甲黏膜相比，鼻息肉中缺氧诱导因子蛋白而非mRNA的表达显著增加，但它与疾病严重程度无关。
结论
我们认为缺氧可能在鼻息肉病的起始过程中起作用。
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