Zhao Chun Tian, Wang En Tao, Chen Wen Feng, Chen Wen Xin
Key Laboratory of Agro-Microbial Resource and Application, Ministry of Agriculture/Department of Microbiology and Immunology, College of Biological Sciences, China Agricultural University, Beijing, China.
FEMS Microbiol Lett. 2008 Sep;286(2):263-73. doi: 10.1111/j.1574-6968.2008.01282.x. Epub 2008 Jul 24.
Based on the analyses of ribosomal DNA and housekeeping genes, a total of 118 bacterial isolates obtained from 13 Astragalus species grown in the temperate region of China were identified as 19 genomic species of Mesorhizobium, Rhizobium, Sinorhizobium and Bradyrhizobium, two of them being putatively new species. Phylogenetic comparison of symbiotic genes (nodC and nifH) and housekeeping genes showed that the symbiotic genes of the Astragalus rhizobia were maintained by both vertical and horizontal transfer. The results demonstrated that the Astragalus species were very promiscuous hosts for rhizobia and that their rhizobia had very diverse genomic and symbiotic gene backgrounds.
基于核糖体DNA和管家基因分析，从中国温带地区种植的13种黄芪属植物中获得的118株细菌分离株被鉴定为中生根瘤菌属、根瘤菌属、中华根瘤菌属和慢生根瘤菌属的19个基因组种，其中两个可能是新物种。共生基因（nodC和nifH）和管家基因的系统发育比较表明，黄芪属根瘤菌的共生基因通过垂直和水平转移得以维持。结果表明，黄芪属植物是根瘤菌非常混杂的宿主，其根瘤菌具有非常多样的基因组和共生基因背景。
