Wang Lei, Deng Jixian, Wang Jian, Xiang Bo, Yang Tonghua, Gruwel Marco, Kashour Tarek, Tomanek Boguslaw, Summer Randy, Freed Darren, Jassal Davinder S, Dai Guangping, Glogowski Miriam, Deslauriers Roxanne, Arora Rakesh C, Tian Ganghong
Institute for Biodiagnostics, National Research Council Canada, Winnipeg, Canada R3B 1Y6.
Magn Reson Imaging. 2009 Jan;27(1):108-19. doi: 10.1016/j.mri.2008.05.013. Epub 2008 Jul 26.
The objectives of this study were (1) to determine whether superparamagnetic iron oxide (SPIO) affects viability, transdifferentiation potential and cell-factor secretion of adipose-derived stem cells (ASCs); and (2) to determine whether SPIO-enhanced magnetic resonance (MR) imaging highlights living stem cells. Rat ASCs were incubated in SPIO-containing cell culture medium for 2 days. The SPIO-treated ASCs were then subjected to adipogenic, osteogenic and myogenic transdifferentiation. Expression of vascular endothelial growth factor, hepatocyte growth factor and insulin-like growth factor 1 by the SPIO-treated ASCs was measured using reverse transcription polymerase chain reaction. Cell viability was assessed using trypan blue stain. For in vivo experiments, SPIO-labeled ASCs were injected into 10 rat hearts. The hearts were monitored using MRI. We found that survival rate of the ASCs cultured in the SPIO-containing medium was very high (97-99%). The SPIO-treated ASCs continued to express specific markers for the three types of transdifferentiation. Expression of the cell factors by the ASCs was not affected by SPIO. Signal voids on MR images were associated with the living SPIO-labeled ASCs in the rat hearts. We conclude that SPIO does not affect viability, transdifferentiation potential or cell-factor secretion of ASCs. MRI mainly highlights living SPIO-labeled stem cells.
（1）确定超顺磁性氧化铁（SPIO）是否会影响脂肪来源干细胞（ASC）的活力、转分化潜能和细胞因子分泌；（2）确定SPIO增强磁共振（MR）成像是否能凸显活的干细胞。将大鼠ASC在含SPIO的细胞培养基中孵育2天。然后对经SPIO处理的ASC进行成脂、成骨和成肌转分化。使用逆转录聚合酶链反应测量经SPIO处理的ASC中血管内皮生长因子、肝细胞生长因子和胰岛素样生长因子1的表达。使用台盼蓝染色评估细胞活力。对于体内实验，将SPIO标记的ASC注射到10只大鼠心脏中。使用MRI监测心脏。我们发现，在含SPIO的培养基中培养的ASC存活率非常高（97 - 99%）。经SPIO处理的ASC继续表达三种转分化类型的特异性标志物。ASC对细胞因子的表达不受SPIO影响。MR图像上的信号缺失与大鼠心脏中活的SPIO标记的ASC相关。我们得出结论，SPIO不影响ASC的活力、转分化潜能或细胞因子分泌。MRI主要凸显活的SPIO标记的干细胞。
