Wang Jian, Xiang Bo, Deng Jixian, Freed Darren H, Arora Rakesh C, Tian Ganghong
Department of Vascular Surgery, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, 1277 Jiefang Street, Wuhan, Hubei 430022, China; National Research Council of Canada, 435 Ellice Avenue, Winnipeg, MB, Canada R3B 1Y6; Department of Physiology, Faculty of Medicine, University of Manitoba, 727 McDermot Avenue, Winnipeg, MB, Canada R3E 3P5.
National Research Council of Canada, 435 Ellice Avenue, Winnipeg, MB, Canada R3B 1Y6; Department of Pharmacology and Therapeutics, Faculty of Medicine, University of Manitoba, 753 McDermot Avenue, Winnipeg, MB, Canada R3E 0T6.
Stem Cells Int. 2016;2016:7168175. doi: 10.1155/2016/7168175. Epub 2016 Jan 6.
After seven-day exposure to 0.5-Tesla Static Magnetic Field (SMF), Adipose-derived Stem Cells (ASCs) and those labeled by superparamagnetic iron oxide (SPIO) nanoparticles were examined for viability by methyl thiazol tetrazolium (MTT) assay, proliferation by cell counting and bromodeoxyuridine (BrdU) incorporation, DNA integrity by single cell gel electrophoresis, surface antigen by flow cytometry analysis, and the expression of cytokines and genetic markers by reverse transcription-PCR and underwent adipogenic and osteogenic differentiation assessed by quantifying related specific genes expression. The SMF slightly reduced cell viability and proliferation and inhibited the expression of CD49d, CD54, and CD73 but did not damage DNA integrity. The SMF slightly downregulated the expression of cytokines including Vascular Endothelial Growth Factor (VEGF), Insulin-like Growth Factor-1 (IGF-1), Transforming Growth Factor Beta 1 (TGF-β1), genetic markers comprising Stem Cell Antigen-1 (Sca1), Octamer-4 (Oct-4), ATP-binding Cassette Subfamily B Member 1 (ABCB1), adipogenic marker genes containing Lipoprotein Lipase (LPL), Peroxisome Proliferator-Activated Receptor Gamma (PPAR-γ), and osteogenic marker genes including Secreted Phosphor-protein 1 (SPP1) and Osterix (OSX). Exposure to 0.5 T SMF for seven days inhibited viability, proliferation, surface antigen expression, cytokine secretion, stem cell genetic marker expression, and adipogenic and osteogenic differentiation but did not affect the DNA integrity in ASCs with or without SPIO labeling.
在将脂肪来源干细胞（ASCs）和用超顺磁性氧化铁（SPIO）纳米颗粒标记的细胞暴露于0.5特斯拉静磁场（SMF）7天后，通过噻唑蓝（MTT）法检测细胞活力，通过细胞计数和溴脱氧尿苷（BrdU）掺入检测细胞增殖，通过单细胞凝胶电泳检测DNA完整性，通过流式细胞术分析检测表面抗原，通过逆转录聚合酶链反应检测细胞因子和遗传标志物的表达，并通过定量相关特定基因的表达评估其成脂和成骨分化。SMF轻微降低了细胞活力和增殖，并抑制了CD49d、CD54和CD73的表达，但未损害DNA完整性。SMF轻微下调了包括血管内皮生长因子（VEGF）、胰岛素样生长因子-1（IGF-1）、转化生长因子β1（TGF-β1）在内的细胞因子的表达，下调了包括干细胞抗原-1（Sca1）、八聚体结合转录因子4（Oct-4）、ATP结合盒转运体B成员1（ABCB1）在内的遗传标志物的表达，下调了包括脂蛋白脂肪酶（LPL）、过氧化物酶体增殖物激活受体γ（PPAR-γ）在内的成脂标志物基因的表达，以及包括分泌型磷蛋白1（SPP1）和osterix（OSX）在内的成骨标志物基因的表达。暴露于0.5 T SMF 7天抑制了细胞活力、增殖、表面抗原表达、细胞因子分泌、干细胞遗传标志物表达以及成脂和成骨分化，但不影响有无SPIO标记的ASCs的DNA完整性。
