Kotakadi Venkata S, Gaddam Susmila Aparna, Venkata Sucharitha K, Sarma P V G K, Sai Gopal D V R
DST-PURSE Centre, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
Department of Virology, Sri Venkateswara University, Tirupati, Andhra Pradesh, India.
3 Biotech. 2016 Dec;6(2):216. doi: 10.1007/s13205-016-0532-5. Epub 2016 Oct 6.
Biosynthesis of plant-mediated silver nanoparticles is gaining significant importance due to environmentally safe 'green method' and it is an efficient alternative method. In the present study, silver nanoparticles were synthesized by using root extract of Glycyrrhiza glabra an important medicinal plant. The AgNPs are characterized by spectral analysis; the surface plasmon resonance (SPR) peak of AgNPs showed maximum absorption at 445 nm. Fourier-transform infrared spectroscopy (FT-IR) data show that the O-H hydroxyl groups, carboxylic acids, ester and ether groups and C-O stretching of alcohols have been utilized in the formation of AgNPs. The X-ray powder diffraction (XRD) data reveal that the AgNPs are face-centered cubic (fcc) in structure. The size was determined by particle size analyzer and atomic force microscope (AFM); the results reveal that AgNPs were spherical in shape and the average grain size is determined as 41.5-46.5 nm. Transmission electron microscopy (TEM) micrographs obtained show that AgNPs were roughly spherical and well dispersed with the sizes ranging from 10 to 45 nm ± 5 nm. The biofabricated AgNPs are extremely stable due to its high negative zeta potential -34.1 mV which indicates that the nanoparticles are polydispered in nature. The cytotoxic studies of AgNPs on human CD34 +ve stem cells in microcarrier culture reveal excellent growth at different concentrations of biosynthesized AgNPs. This is the first report of microcarrier culture of CD34 +ve stem cells on biosynthesized AgNPs.
由于环境安全的“绿色方法”，植物介导的银纳米颗粒的生物合成正变得越来越重要，并且它是一种有效的替代方法。在本研究中，使用重要的药用植物光果甘草的根提取物合成了银纳米颗粒。通过光谱分析对银纳米颗粒进行了表征；银纳米颗粒的表面等离子体共振（SPR）峰在445nm处显示出最大吸收。傅里叶变换红外光谱（FT-IR）数据表明，O-H羟基、羧酸、酯和醚基团以及醇的C-O伸缩振动已用于银纳米颗粒的形成。X射线粉末衍射（XRD）数据表明，银纳米颗粒的结构为面心立方（fcc）。通过粒度分析仪和原子力显微镜（AFM）确定了尺寸；结果表明，银纳米颗粒呈球形，平均粒径确定为41.5-46.5nm。获得的透射电子显微镜（TEM）显微照片表明，银纳米颗粒大致呈球形且分散良好，尺寸范围为10至45nm±5nm。生物制造的银纳米颗粒由于其高负zeta电位-34.1mV而极其稳定，这表明纳米颗粒在本质上是多分散的。在微载体培养中，银纳米颗粒对人CD34 +ve干细胞的细胞毒性研究表明，在不同浓度的生物合成银纳米颗粒下具有良好的生长。这是关于在生物合成的银纳米颗粒上进行CD34 +ve干细胞微载体培养的首次报道。
