Becher D, Wedler H, Schulze H, Bode R, Kasüske A, Samsonova I
Fachrichtung Biologie, Ernst-Moritz-Arndt-Universität Greifswald, FRG.
Mol Gen Genet. 1991 Jul;227(3):361-8. doi: 10.1007/BF00273924.
The four enzymatic steps in the conversion of alpha-ketoisovaleriate to leucine were examined in the wild type and in 13 leucine auxotrophic strains of Candida maltosa. The genetic lesions in the auxotrophs, involve at least five different loci and are correlated with three enzymatic steps. This was confirmed by gene cloning, protoplast fusion, and enzyme assays. The pathway for leucine biosynthesis in C. maltosa shows general similarity to that of other lower eukaryotes but there are individual differences in the numbers of genes responsible for single enzymatic steps. A disomic state of the chromosomes carrying genes coding for alpha-isopropylmalate synthase and beta-isopropyl-malate dehydrogenase was elucidated.
在麦芽糖假丝酵母的野生型菌株以及13种亮氨酸营养缺陷型菌株中，研究了α-酮异戊酸转化为亮氨酸的四个酶促步骤。营养缺陷型菌株中的遗传损伤至少涉及五个不同的基因座，并且与三个酶促步骤相关。这通过基因克隆、原生质体融合和酶分析得到了证实。麦芽糖假丝酵母中亮氨酸生物合成途径与其他低等真核生物的途径总体相似，但在负责单个酶促步骤的基因数量上存在个体差异。阐明了携带编码α-异丙基苹果酸合酶和β-异丙基苹果酸脱氢酶基因的染色体的二体状态。
