Silvany R E, Dougherty J M, McCulley J P
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas 75235-9057.
Ophthalmology. 1991 Jun;98(6):854-7. doi: 10.1016/s0161-6420(91)32210-3.
Single preservatives used in contact lens solutions were evaluated for their effectiveness in killing Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites and cysts. Preservatives were tested against amoebae at intervals varying from 30 minutes to 24 hours. The preservatives were tested with axenically and nonaxenically grown organisms. Chlorhexidine (0.001% and 0.005%), polyaminopropyl biguanide (0.0015%), benzalkonium chloride (0.001% and 0.004%), and hydrogen peroxide (3%) were very effective preservatives. Lower concentrations of these same preservatives were less effective. Thimerosal (0.001% and 0.004%), sorbic acid (0.1%), potassium sorbate (0.13%), EDTA (0.1%), polyaminopropyl biguanide (0.00005%), and polyquaternium-1 (0.001%) were not effective as tested. However, thimerosal 0.004% when combined in solution with EDTA was effective. Preservatives were more effective when tested against axenically prepared organisms than when tested against co-cultured organisms.
对隐形眼镜护理液中使用的单一防腐剂进行了评估，以确定其对杀灭卡氏棘阿米巴和多食棘阿米巴滋养体及包囊的有效性。在30分钟至24小时不等的时间间隔内对防腐剂进行了抗阿米巴测试。使用无菌培养和非无菌培养的生物体对防腐剂进行了测试。洗必泰（0.001%和0.005%）、聚氨基丙基双胍（0.0015%）、苯扎氯铵（0.001%和0.004%）和过氧化氢（3%）是非常有效的防腐剂。这些相同防腐剂的较低浓度效果较差。硫柳汞（0.001%和0.004%）、山梨酸（0.1%）、山梨酸钾（0.13%）、乙二胺四乙酸（0.1%）、聚氨基丙基双胍（0.00005%）和聚季铵盐-1（0.001%）经测试无效。然而，0.004%的硫柳汞与乙二胺四乙酸混合在溶液中时是有效的。与针对共培养生物体进行测试相比，防腐剂在针对无菌制备的生物体进行测试时更有效。
