Silvany R E, Dougherty J M, McCulley J P, Wood T S, Bowman R W, Moore M B
Department of Ophthalmology, University of Texas Southwestern Medical Center, Dallas.
Ophthalmology. 1990 Mar;97(3):286-90. doi: 10.1016/s0161-6420(90)32590-3.
Contact lens disinfection systems were evaluated for their effectiveness in killing Acanthamoeba castellanii and Acanthamoeba polyphaga trophozoites and cysts. Amoebae were inoculated into commercially available contact lens cleaning and soaking solutions. At intervals varying from 30 minutes to 24 hours, solutions were filtered. The filters were removed and cultured for Acanthamoeba organisms. Striking differences were observed in the abilities of the different disinfecting solutions to kill the organisms. Solutions containing chlorhexidine were effective at very short exposure times. Solutions containing benzalkonium chloride required slightly longer exposure times but were faster than solutions containing only thimerosal. Solutions containing sorbate, polyaminopropyl biguanide, or polyquaternium-1 were not effective at killing Acanthamoeba organisms in the time allotted for the experiment. Solutions containing hydrogen peroxide were quite effective if the agent was not prematurely catalyzed. A. polyphaga generally required longer exposure to disinfectants than did A. castellanii for complete inhibition to occur.
对隐形眼镜消毒系统杀灭卡氏棘阿米巴和多食棘阿米巴滋养体及包囊的有效性进行了评估。将阿米巴接种到市售的隐形眼镜清洁和浸泡溶液中。在30分钟至24小时不等的时间间隔后，对溶液进行过滤。取出滤器并培养棘阿米巴生物体。观察到不同消毒溶液杀灭生物体的能力存在显著差异。含氯己定的溶液在极短的暴露时间内就有效。含苯扎氯铵的溶液需要稍长的暴露时间，但比仅含硫柳汞的溶液更快。含山梨酸盐、聚氨基丙基双胍或聚季铵盐-1的溶液在实验规定的时间内对杀灭棘阿米巴生物体无效。如果过氧化氢不被过早催化，含过氧化氢的溶液相当有效。一般来说，多食棘阿米巴比卡氏棘阿米巴需要更长时间暴露于消毒剂才能完全被抑制。
