Nauheim R C, Brockman R J, Stopak S S, Turgeon P W, Keleti G, Roat M I, Thoft R A
Eye & Ear Institute of Pittsburgh, Department of Ophthalmology, University of Pittsburgh, PA 15213.
Cornea. 1990 Oct;9(4):290-3.
Acanthamoeba may cause a severe keratitis in contact lens wearers. Since most sterilization techniques require rinsing the lenses prior to insertion, contaminated solutions represent a potential vector for transmission of Acanthamoeba. The ability of rinse solutions to sustain an inoculum of Acanthamoeba polyphaga was investigated. A. polyphaga was exposed to 0.1% benzalkonium chloride, 0.001% thimerosal/0.1% edetate disodium, 0.1% edetate disodium, saline, tap water, and distilled water. The status of the organism was evaluated with direct microscopic counts and cultures to confirm viability. Incubation with 0.1% edetate disodium, saline, tap water, and distilled water resulted in the maintenance of reduced populations of viable organisms for 7 days. Benzalkonium chloride preserved saline and solutions containing thimerosal with edetate rendered the Acanthamoeba nonviable.
棘阿米巴可导致隐形眼镜佩戴者发生严重的角膜炎。由于大多数消毒技术要求在插入镜片前冲洗镜片，受污染的溶液是棘阿米巴传播的潜在载体。研究了冲洗液维持多食棘阿米巴接种物的能力。将多食棘阿米巴暴露于0.1%苯扎氯铵、0.001%硫柳汞/0.1%乙二胺四乙酸二钠、0.1%乙二胺四乙酸二钠、生理盐水、自来水和蒸馏水中。通过直接显微镜计数和培养评估生物体的状态以确认其活力。与0.1%乙二胺四乙酸二钠、生理盐水、自来水和蒸馏水一起孵育导致存活生物体数量减少并维持7天。苯扎氯铵保存的生理盐水以及含有硫柳汞和乙二胺四乙酸的溶液可使棘阿米巴失去活力。
