Pezzi L, Marquez C, Toribio M L, Martinez C
Centro de Biologia Molecular CSIC, Universidad Autonoma de Madrid.
Res Immunol. 1991 Feb;142(2):109-15. doi: 10.1016/0923-2494(91)90018-e.
The association of alkaline phosphatase (ALPase) with the cytoskeleton in lymphoid cells was investigated. Extracting cells with non-ionic detergents such as Triton, we determined that ALPase is present in the cytoskeletal fraction in fully differentiated B lymphocytes, X63 myeloma cells and Sp2/O hybridoma cells. During the course of B-lymphocyte activation, the ALPase shifted from a soluble to a Triton-insoluble form. Changes in the phosphorylation of Triton-insoluble proteins with molecular weights of 120, 100, 90, 75, 34 and 31 kDa were detected, coinciding with the appearance of the ALPase in this fraction. The possible role of ALPase in the differentiation of B cells is discussed.
研究了碱性磷酸酶（ALPase）与淋巴细胞细胞骨架的关联。用非离子去污剂如 Triton 提取细胞，我们确定在完全分化的 B 淋巴细胞、X63 骨髓瘤细胞和 Sp2/O 杂交瘤细胞的细胞骨架部分中存在 ALPase。在 B 淋巴细胞激活过程中，ALPase 从可溶形式转变为 Triton 不溶形式。检测到分子量为 120、100、90、75、34 和 31 kDa 的 Triton 不溶蛋白的磷酸化变化，这与该部分中 ALPase 的出现一致。讨论了 ALPase 在 B 细胞分化中的可能作用。
