Valkiūnas Gediminas, Iezhova Tatjana A, Loiseau Claire, Chasar Anthony, Smith Thomas B, Sehgal Ravinder N M
Institute of Ecology, Vilnius University, Akademijos 2, Vilnius, 08412, Lithuania.
Parasitol Res. 2008 Oct;103(5):1213-28. doi: 10.1007/s00436-008-1118-x. Epub 2008 Jul 31.
Plasmodium (Novyella) megaglobularis n. sp. was recorded in the olive sunbird Cyanomitra olivacea, and Plasmodium (Novyella) globularis n. sp. and Haemoproteus (Parahaemoproteus) vacuolatus n. sp. were found in the yellow-whiskered greenbul Andropadus latirostris in rainforests of Ghana and Cameroon. These parasites are described based on the morphology of their blood stages and a segment of the mitochondrial cytochrome b gene, which can be used for molecular identification and diagnosis of these species. Illustrations of blood stages of new species are given, and phylogenetic analysis identifies deoxyribonucleic acid (DNA) lineages closely related to these parasites. Traditional taxonomy of avian pigment-forming haemosporidians of the families Plasmodiidae and Haemoproteidae is discussed based on the recent molecular phylogenies of these parasites. We conclude that further work to increase the number of precise linkages between haemosporidian DNA sequences and their corresponding morphospecies is needed before revising the current classification of haemosporidians. This study emphasises the value of both the polymerase chain reaction and microscopy in the identification of avian haemosporidian parasites.
在橄榄太阳鸟（Cyanomitra olivacea）中发现了巨球疟原虫（诺维耶疟原虫）新种（Plasmodium (Novyella) megaglobularis n. sp.），在加纳和喀麦隆雨林中的黄须绿鸫（Andropadus latirostris）中发现了球疟原虫（诺维耶疟原虫）新种（Plasmodium (Novyella) globularis n. sp.）和空泡血变虫（副血变虫）新种（Haemoproteus (Parahaemoproteus) vacuolatus n. sp.）。这些寄生虫是根据其血液阶段的形态以及线粒体细胞色素b基因的一段序列进行描述的，该序列可用于这些物种的分子鉴定和诊断。给出了新物种血液阶段的图示，系统发育分析确定了与这些寄生虫密切相关的脱氧核糖核酸（DNA）谱系。基于这些寄生虫最近的分子系统发育，讨论了疟原虫科和血变虫科鸟类色素形成血孢子虫的传统分类。我们得出结论，在修订当前血孢子虫分类之前，需要进一步开展工作以增加血孢子虫DNA序列与其相应形态物种之间精确联系的数量。本研究强调了聚合酶链反应和显微镜检查在鉴定鸟类血孢子虫寄生虫方面的价值。
