Nimmo Wilkie J S, Yager J A, Wilkie B N, Parker W M
Department of Pathology, Ontario Veterinary College, University of Guelph, Canada.
Vet Immunol Immunopathol. 1991 Apr;28(2):97-106. doi: 10.1016/0165-2427(91)90132-v.
Antigen specific and nonspecific T-lymphocyte activity was evaluated in normal dogs and in dogs with atopic dermatitis by measuring the increase in skin thickness after application of the contact allergen dinitrochlorobenzene and after intradermal injection of the mitogens phytohemagglutinin and concanavalin A. The atopic dogs had a significantly reduced response to the contact allergen (P less than or equal to 0.001) but a significantly increased response to the mitogens (P less than or equal to 0.001). The atopic and normal dogs responded similarly to intradermally injected histamine. The response of dogs with non-atopic skin conditions to the cutaneous mitogen test was like that of normal dogs. Pre-existing dermatitis does not apparently influence cutaneous response to mitogens in dogs. The cutaneous response of atopics during treatment with corticosteroids is not different from normal controls. These results suggest a role for altered cell-mediated immunity in the pathogenesis of canine atopy and that the cutaneous mitogen test may have value as a rapid screening test for the disease.
通过测量接触性变应原二硝基氯苯应用后以及皮内注射有丝分裂原植物血凝素和刀豆球蛋白A后皮肤厚度的增加，评估正常犬和特应性皮炎犬的抗原特异性和非特异性T淋巴细胞活性。特应性犬对接触性变应原的反应显著降低（P≤0.001），但对有丝分裂原的反应显著增加（P≤0.001）。特应性犬和正常犬对皮内注射组胺的反应相似。非特应性皮肤状况的犬对皮肤有丝分裂原试验的反应与正常犬相似。先前存在的皮炎显然不会影响犬对有丝分裂原的皮肤反应。用皮质类固醇治疗期间特应性犬的皮肤反应与正常对照无差异。这些结果表明细胞介导的免疫改变在犬特应性发病机制中起作用，并且皮肤有丝分裂原试验可能作为该疾病的快速筛查试验具有价值。
