Panasyuk George Y, Wang Zheng-Min, Schotland John C, Markel Vadim A
Department of Bioengineering, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA.
Opt Lett. 2008 Aug 1;33(15):1744-6. doi: 10.1364/ol.33.001744.
In recent years, optical tomography (OT) of highly scattering biological samples has increasingly relied on noncontact CCD-based imaging devices that can record extremely large data sets, with up to 10(9) independent measurements per sample. Reconstruction of such data sets requires fast algorithms. The latter have been developed and applied experimentally in our previous work to imaging of the intrinsic absorption coefficient of highly scattering media. However, it is widely recognized that the use of fluorescent contrast agents in OT has the potential to significantly enhance the technique. We show that the algorithms previously developed by us can be modified to reconstruct the concentration of fluorescent contrast agents.
近年来，对高散射生物样本的光学层析成像（OT）越来越依赖于基于非接触式电荷耦合器件（CCD）的成像设备，这些设备能够记录极大的数据集，每个样本多达10⁹次独立测量。重建这样的数据集需要快速算法。在我们之前的工作中，已经开发了这些算法并将其应用于高散射介质固有吸收系数的成像实验中。然而，人们普遍认识到，在光学层析成像中使用荧光造影剂有可能显著提升该技术。我们表明，我们之前开发的算法可以进行修改，以重建荧光造影剂的浓度。
