Atmar V T, Throneberry G O, Kuehn G D
Mycopathologia. 1976 Oct 22;59(3):171-4. doi: 10.1007/BF00627879.
Growth of Verticillium albo-atrum in liquid Czapek-Dox broth was stimulated about four-fold by added 10 mM adenine, N6-benzyladenine, or kinetin. Less stimulation was evident at lower concentrations. With none of these included in the basal growth medium, detectable protein kinase activity in cell-free extracts was low and responded minimally to cAMP (adenosine 3',5'-cyclic monophosphate) in the reaction mixture. With each of these compounds as an additive to the growth medium, protein kinase activity was not only greater but also responded markedly (several fold) to cAMP. These results demonstrate a strong correlation between exogenously supplied adenine and related compounds, increased growth, increased protein kinase activity, and kinase response to cAMP in this organism.
在液体察氏培养基中，添加10 mM腺嘌呤、N6-苄基腺嘌呤或激动素可使黑白轮枝菌的生长受到约四倍的刺激。较低浓度时刺激作用不明显。在基础生长培养基中不添加这些物质时，无细胞提取物中可检测到的蛋白激酶活性较低，并且对反应混合物中的环磷酸腺苷（cAMP）反应极小。当将这些化合物中的每一种作为生长培养基的添加剂时，蛋白激酶活性不仅更高，而且对cAMP的反应也显著增强（几倍）。这些结果表明，在该生物体中，外源供应的腺嘌呤及相关化合物、生长增加、蛋白激酶活性增加以及激酶对cAMP的反应之间存在很强的相关性。
