Bhattacharjee P S, Naylor C J, Jones R C
University of Liverpool, Department of Veterinary Pathology, Neston, South Wirral, UK.
Avian Pathol. 1994 Sep;23(3):471-80. doi: 10.1080/03079459408419017.
A method is described for the simple application of immunofluorescence (IF) staining for the identification of infectious bronchitis virus (IBV) in tracheal organ cultures (TOC). It involves the application of antiserum and fluorescent antiglobulin to unfixed TOC. In TOC inoculated with high titres of virus, specific fluorescence was observed in less than 24 h. IF staining almost always occurred before ciliostasis. Following experimental infection of chicks with IBV, virus could sometimes be detected in material from tracheal or cloacal swabs within 24 h of inoculation of TOC.
描述了一种用于在气管器官培养物（TOC）中鉴定传染性支气管炎病毒（IBV）的免疫荧光（IF）染色简单应用方法。该方法包括将抗血清和荧光抗球蛋白应用于未固定的TOC。在接种高滴度病毒的TOC中，不到24小时就观察到特异性荧光。IF染色几乎总是在纤毛静止之前出现。在用IBV对雏鸡进行实验性感染后，有时在接种TOC后24小时内可从气管或泄殖腔拭子材料中检测到病毒。
