Shamsaddini-Bafti Mehrdad, Vasfi-Marandi M, Momayez R, Toroghi R, Pourbakhsh S A, Salari R, Tabrizchi H
1Razi Vaccine & Serum Research Institute, Kerman Branch, km. 17th of Joupar, Kerman Rd, Kerman, Iran Post. Box: 76175-359.
2Faculty of Veterinary Medicine, University of Tehran, Tehran, Iran.
Comp Clin Path. 2014;23(2):347-352. doi: 10.1007/s00580-012-1621-4. Epub 2012 Sep 29.
The indirect immunoperoxidase (IIP) assay was compared with the reverse transcription-polymerase chain reaction (RT-PCR) for detection of 793/B serotype of infectious bronchitis virus in tissues samples collected from experimentally infected chickens. This technique was optimized in specific pathogen-free (SPF)-embryonated chicken eggs and broiler chickens inoculated with the Iranian IR/773/2001 strain of 793/B serotype The trachea, lung, kidney, and cecal tonsil tissue samples from experimentally infected chicken embryos and chickens were collected in order to prepare tissue sections in IIP assay and to detect in RT-PCR. The sensitivity and specificity values of IIP assay were, respectively, 83 and 84 %, and the positive and negative prediction values were 71 and 91 % when compared with RT-PCR.
采用间接免疫过氧化物酶（IIP）检测法与逆转录-聚合酶链反应（RT-PCR），对从实验感染鸡采集的组织样本中传染性支气管炎病毒793/B血清型进行检测。该技术在特定病原体-free（SPF）鸡胚和接种伊朗793/B血清型IR/773/2001毒株的肉鸡中进行了优化。采集实验感染鸡胚和鸡的气管、肺、肾和盲肠扁桃体组织样本，用于IIP检测法制备组织切片以及RT-PCR检测。与RT-PCR相比，IIP检测法的敏感性和特异性值分别为83%和84%，阳性和阴性预测值分别为71%和91%。
