Wang H N, Wu Q Z, Huang Y, Liu P
College of Animal Science and Technology, Sichuan Agricultural University, Yaan, China.
Avian Dis. 1997 Apr-Jun;41(2):279-82.
A nonhemagglutinating virus was isolated from kidneys and lungs of chickens suspected of having infectious bronchitis infection. Specific-pathogen-free embryonated chicken eggs were used as the cultural system. With the use of the ciliary activity of chicken embryo tracheal organ cultures as indicator system, the physicochemical properties of one of the isolated strains (SAIB3) were shown to be similar to infectious bronchitis virus (IBV) strain M41 (standard strain); whereas electron microscopy of the isolate showed coronavirus particles. Virus-neutralization tests were performed in tracheal organ cultures to compare the serotypes of five IBV isolates and six known IBV strains on the basis of reciprocal neutralization titers and euclidean distance. The cross-neutralization pattern indicated that one isolate was of the T-strain IBV serotype, another of the M41 IBV serotype, while others had partial serotype relationship to M41 and T-strains of IBV.
从疑似感染传染性支气管炎的鸡的肾脏和肺中分离出一种非血凝性病毒。使用无特定病原体的鸡胚作为培养系统。以鸡胚气管器官培养物的纤毛活性作为指示系统，其中一个分离株（SAIB3）的理化特性显示与传染性支气管炎病毒（IBV）毒株M41（标准毒株）相似；而该分离株的电子显微镜检查显示有冠状病毒颗粒。在气管器官培养物中进行病毒中和试验，根据相互中和效价和欧氏距离比较了5株IBV分离株和6株已知IBV毒株的血清型。交叉中和模式表明，一个分离株属于T株IBV血清型，另一个属于M41 IBV血清型，而其他分离株与IBV的M41和T株有部分血清型关系。
