Byers H R, Etoh T, Doherty J R, Sober A J, Mihm M C
Department of Pathology, Harvard Medical School, Massachusetts General Hospital, Boston 02129.
Am J Pathol. 1991 Aug;139(2):423-35.
Random cell migration and actin organization in seven human primary, recurrent cutaneous, and metastatic melanoma cell lines were studied by time-lapse video recording and image analysis. The migration of over 800 randomly selected cells from the cell lines were recorded using an inverted microscope with an attached incubator housing. The fraction of cells with random migration rates greater than 10 microns/hour was 8% in an established primary melanoma cell line, 2% and 34% in two recurrent cutaneous melanoma cell lines, and 5%, 30%, 31%, and 60% in four metastatic cell lines. The three metastatic cell lines with significantly higher mean migration rates (P less than 0.001) were derived from lymph node metastases, whereas the fourth metastatic cell line was derived from a visceral metastasis. The cellular morphology and presence of cell nests in the original tissue correlated with in vitro cell morphology and the formation of colonies. The ability of cells to organize actin into stress fibers directly correlated with significantly higher random migration rates and lack of colony formation. Characterization of random migration rates and actin organization of human melanoma cells that are isolated from different stages of tumor progression may lend insight into metastasis.
通过延时视频记录和图像分析，研究了七种人原发性、复发性皮肤和转移性黑色素瘤细胞系中的随机细胞迁移和肌动蛋白组织。使用带有附属培养箱的倒置显微镜记录了来自这些细胞系的800多个随机选择细胞的迁移情况。在一个已建立的原发性黑色素瘤细胞系中，随机迁移速率大于10微米/小时的细胞比例为8%，在两个复发性皮肤黑色素瘤细胞系中分别为2%和34%，在四个转移性细胞系中分别为5%、30%、31%和60%。平均迁移速率显著更高（P小于0.001）的三个转移性细胞系源自淋巴结转移，而第四个转移性细胞系源自内脏转移。原始组织中的细胞形态和细胞巢的存在与体外细胞形态和集落形成相关。细胞将肌动蛋白组织成应力纤维的能力与显著更高的随机迁移速率和缺乏集落形成直接相关。对从肿瘤进展不同阶段分离的人黑色素瘤细胞的随机迁移速率和肌动蛋白组织进行表征，可能有助于深入了解转移。
