从正常人黑素细胞培养物中选择性去除成纤维细胞。
Selective elimination of fibroblasts from cultures of normal human melanocytes.
作者信息
Halaban R, Alfano F D
出版信息
In Vitro. 1984 May;20(5):447-50. doi: 10.1007/BF02619590.
PMID:6724622
Abstract
The main obstacle to establishing pure normal human melanocytes in vitro is contamination of the cultures by fibroblasts. The obstacle can be overcome by selective destruction of fibroblasts with geneticin ( G418 sulfate). Treatment of mixed cultures with this drug at a concentration of 100 micrograms/ml for two days results in pure cultures of normal human melanocytes.
摘要
在体外建立纯正常人黑素细胞的主要障碍是成纤维细胞对培养物的污染。通过用遗传霉素（硫酸G418）选择性破坏成纤维细胞可以克服这一障碍。以100微克/毫升的浓度用这种药物处理混合培养物两天，可得到纯的正常人黑素细胞培养物。
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