Hansman Grant S, Oka Tomoichiro, Takeda Naokazu
Department of Virology II, National Institute of Infectious Diseases, Japan.
Virus Res. 2008 Nov;137(2):261-5. doi: 10.1016/j.virusres.2008.07.002. Epub 2008 Aug 15.
We expressed full-length sapovirus genome constructs in insect cells and analyzed their products. The capsid protein was cleaved from the ORF1 polyprotein from a native-like genome construct and two full-length genome constructs with mutations in an active polymerase motif, whereas the capsid protein was not cleaved from a full-length genome construct with a mutation in an active protease motif. Our results showed that the sapovirus protease-polymerase precursor protein cleaved the capsid protein from the polyprotein at the putative conserved capsid start. Importantly, the cleaved capsid protein formed empty virus-like particles that were morphologically and antigenically similar to native sapovirus.
我们在昆虫细胞中表达了全长萨波病毒基因组构建体并分析了其产物。衣壳蛋白从天然样基因组构建体以及在活性聚合酶基序中有突变的两个全长基因组构建体的ORF1多聚蛋白上被切割下来，而衣壳蛋白未从在活性蛋白酶基序中有突变的全长基因组构建体上被切割下来。我们的结果表明，萨波病毒蛋白酶-聚合酶前体蛋白在假定的保守衣壳起始位点从多聚蛋白上切割下衣壳蛋白。重要的是，切割后的衣壳蛋白形成了形态和抗原性与天然萨波病毒相似的空病毒样颗粒。
