Ganova-Raeva Lilia, Smith Alvin W, Fields Howard, Khudyakov Yury
Division of Viral Hepatitis, Centers for Disease Control and Prevention, National Center for Infectious Diseases, 1600 Clifton Road NE, MS A-33 Atlanta, GA 30333, USA.
Virus Res. 2004 Jun 15;102(2):207-13. doi: 10.1016/j.virusres.2004.01.033.
The nucleotide sequence and genome organization of a new member of Caliciviridae was determined. Cell culture inoculated with fecal matter from walrus was used to recover fragments of a new virus by Suppression Subtractive Hybridization (SSH). The isolate was identified as a member of the Vesivirus genus of Caliciviridae and designated the name Walrus Calicivirus (WCV). Sets of PCR primers spanning the entire putative genome were designed using known sequences of other vesiviruses. The assembled genome was 8289 nucleotides (nt) long and shared no more than 87% identity with sequences of the other members of the genus Vesivirus. The largest open reading frame (ORF1) between positions 4-5646 encoded a polyprotein. ORF2, found at position 5652-7778, encoded a putative capsid protein. ORF3 overlapped ORF2 and encoded a small basic protein. Comparative analysis of multiple caliciviral capsid proteins was performed to propose a uniform capsid structural organization for this viral family.
确定了杯状病毒科一个新成员的核苷酸序列和基因组结构。用海象粪便接种细胞培养物，通过抑制性消减杂交（SSH）来获得一种新病毒的片段。该分离株被鉴定为杯状病毒科水疱疹病毒属的成员，并命名为海象杯状病毒（WCV）。利用其他水疱疹病毒的已知序列设计了覆盖整个假定基因组的PCR引物组。组装后的基因组长度为8289个核苷酸（nt），与水疱疹病毒属其他成员的序列同一性不超过87%。4至5646位之间最大的开放阅读框（ORF1）编码一个多聚蛋白。位于5652至7778位的ORF2编码一个假定的衣壳蛋白。ORF3与ORF2重叠，编码一个小的碱性蛋白。对多种杯状病毒衣壳蛋白进行了比较分析，以提出该病毒家族统一的衣壳结构组织。
