Akamatsu H, Komura J, Asada Y, Miyachi Y, Niwa Y
Department of Dermatology, Kansai Medical University.
Arch Dermatol Res. 1991;283(3):162-6. doi: 10.1007/BF00372056.
It has been shown that acne, hyperpigmentation and lentigo malignant are more or less related pathogenetically to reactive oxygen species (ROS). It has recently been reported that azelaic acid is effective in treating these conditions and that it possesses anti-enzymatic and antimitochondrial activity, including cytochrome-P450 reductase and 5 alpha-reductase in microsomal preparations with nicotinamide adenine dinucleotide phosphate (NADPH). We therefore investigated the effects of azelaic acid on human neutrophil functions, such as chemotaxis, phagocytosis and ROS generation. ROS generation in a cell-free system was also assessed. The results revealed that neutrophil chemotaxis and phagocytosis as well as ROS generated in a xanthine-xanthine-oxidase system were not significantly changed in the presence of azelaic acid. However, azelaic acid markedly decreased O2- and OH. generated by neutrophils. It may be concluded that the reported clinical effectiveness of azelaic acid is partly due to its inhibitory action on neutrophil-generated ROS, leading to a reduction both in oxidative tissue injury at sites of inflammation and in melanin formation.
研究表明，痤疮、色素沉着过度和恶性雀斑样痣在发病机制上或多或少与活性氧（ROS）有关。最近有报道称壬二酸对治疗这些病症有效，且具有抗酶和抗线粒体活性，包括在含有烟酰胺腺嘌呤二核苷酸磷酸（NADPH）的微粒体制剂中对细胞色素P450还原酶和5α - 还原酶的抑制作用。因此，我们研究了壬二酸对人类中性粒细胞功能的影响，如趋化性、吞噬作用和ROS生成。还评估了无细胞系统中的ROS生成情况。结果显示，在壬二酸存在的情况下，中性粒细胞趋化性、吞噬作用以及黄嘌呤 - 黄嘌呤氧化酶系统中产生的ROS没有显著变化。然而，壬二酸显著降低了中性粒细胞产生的超氧阴离子（O2-）和羟基自由基（OH·）。可以得出结论，壬二酸已报道的临床疗效部分归因于其对中性粒细胞产生的ROS的抑制作用，从而减少了炎症部位的氧化组织损伤和黑色素形成。
