Faye Oumar, Faye Ousmane, Dupressoir Anne, Weidmann Manfred, Ndiaye Mady, Alpha Sall Amadou
Institut Pasteur de Dakar, 36 Avenue Pasteur, BP 220, Dakar, Senegal.
J Clin Virol. 2008 Sep;43(1):96-101. doi: 10.1016/j.jcv.2008.05.005. Epub 2008 Jul 31.
Zika virus (ZIKV) is an emerging mosquito-borne flavivirus circulating in Asia and Africa. Human infection induces an influenza-like syndrome that is associated with retro-orbital pain, oedema, lymphadenopathy, or diarrhea. Diagnosis of Zika fever requires virus isolation and serology, which are time consuming or cross-reactive.
To develop a one-step RT-PCR assay to detect ZIKV in human serum.
An assay targeting the envelope protein coding region was designed and evaluated for its specificity, detection limit, repeatability, and capacity to detect ZIKV isolates collected over a 40-year period from various African countries and hosts.
The assay's detection limit and repeatability were respectively 7.7pfu/reaction and 100% in serum and L-15 medium; none of 19 other flaviviruses tested were detected.
The assay is rapid, sensitive, and specific to detect ZIKV in cell culture or serum, but needs to be validated for diagnosis using clinical samples.
寨卡病毒（ZIKV）是一种新出现的经蚊子传播的黄病毒，在亚洲和非洲传播。人类感染会引发类似流感的综合征，伴有眼眶后疼痛、水肿、淋巴结病或腹泻。寨卡热的诊断需要病毒分离和血清学检测，这些方法耗时或存在交叉反应。
开发一种一步法逆转录聚合酶链反应（RT-PCR）检测方法，用于检测人血清中的寨卡病毒。
设计了一种针对包膜蛋白编码区的检测方法，并对其特异性、检测限、重复性以及检测从不同非洲国家和宿主收集的40年间的寨卡病毒分离株的能力进行了评估。
该检测方法在血清和L-15培养基中的检测限和重复性分别为7.7个噬斑形成单位/反应和100%；所检测的19种其他黄病毒均未被检测到。
该检测方法在细胞培养物或血清中检测寨卡病毒快速、灵敏且特异，但需要使用临床样本进行诊断验证。
