Abdul-Careem M F, Hunter B D, Lee L F, Fairbrother J H, Haghighi H R, Read L, Parvizi P, Heidari M, Sharif S
Department of Pathobiology, Ontario Veterinary College, University of Guelph, Guelph, Ontario, Canada.
Virology. 2008 Sep 30;379(2):256-65. doi: 10.1016/j.virol.2008.06.027.
The bursa of Fabricius serves as an important tissue in the process of Marek's disease virus (MDV) pathogenesis, since B cells of the bursa harbor the cytolytic phase of MDV replication cycle. In the present study, host responses associated with MDV infection in the bursa of Fabricius of chickens were investigated. The expression of MDV phosphoprotein (pp)38 antigen, MDV glycoprotein (gB) and MDV viral interleukin (vIL)-8 transcripts was at the highest at 4 days post-infection (d.p.i.) and then showed a declining trend. On the contrary, the expression of meq (MDV EcoRI Q) gene as well as the viral genome load increased gradually until day 14 post-infection. The changes in viral parameters were associated with significantly higher infiltration of macrophages and T cell subsets, particularly CD4+ T cells into the bursa of Fabricius. Of the genes examined, the expression of interferon (IFN)-alpha, IFN-gamma genes and inducible nitric oxide synthase (iNOS) was significantly up-regulated in response to MDV infection in the bursa of Fabricius. The results suggest a role for these cells and cytokines in MDV-induced responses in the bursa of Fabricius.
法氏囊是马立克氏病病毒（MDV）致病过程中的重要组织，因为法氏囊中的B细胞承载着MDV复制周期的细胞溶解阶段。在本研究中，对鸡法氏囊中与MDV感染相关的宿主反应进行了研究。MDV磷蛋白（pp）38抗原、MDV糖蛋白（gB）和MDV病毒白细胞介素（vIL）-8转录本的表达在感染后4天（d.p.i.）时最高，然后呈下降趋势。相反，meq（MDV EcoRI Q）基因的表达以及病毒基因组载量在感染后第14天之前逐渐增加。病毒参数的变化与巨噬细胞和T细胞亚群，特别是CD4+ T细胞向法氏囊的显著更高浸润有关。在所检测的基因中，干扰素（IFN）-α、IFN-γ基因和诱导型一氧化氮合酶（iNOS）的表达在法氏囊中对MDV感染的反应中显著上调。结果表明这些细胞和细胞因子在MDV诱导的法氏囊反应中发挥作用。
