Adone R, Francia M, Ciuchini F
Dipartimento di Sanità Alimentare ed Animale, Istituto Superiore di Sanità, Rome, Italy.
Vaccine. 2008 Sep 8;26(38):4913-7. doi: 10.1016/j.vaccine.2008.07.030. Epub 2008 Aug 15.
Brucella melitensis strain Rev1 is used as vaccine for the prophylaxis of brucellosis in sheep and goats. Because of its smooth phenotype, however, it induces antibodies directed to the O-polysaccharide (O-PS) of the lipopolysaccharide (LPS), thus unabling to distinguish between vaccinated and infected animals. It has been speculated that alternative vaccines could be live, attenuated Brucella rough strains, which are devoid of the O-PS. B. melitensis B115 is a natural, attenuated, rough strain. The O-PS is not exposed at the surface but is present in the cytoplasm. We tested the protective activity of B115 against B. melitensis and B. ovis infections in mice, in comparison with that of Rev1. The residual virulence and the humoral response following B115 vaccination were also evaluated. Vaccination with B115 conferred significant protective immunity against B. melitensis 16M and B. ovis challenge strains, equivalent to that provided by Rev1. No interfering antibodies to O-PS were detected, while the B115 vaccination was monitored by a specific B115-based complement fixation test. These promising features suggest further evaluation of B. melitensis B115 as vaccine for target animal hosts.
布鲁氏菌 melitensis 菌株 Rev1 被用作预防绵羊和山羊布鲁氏菌病的疫苗。然而，由于其光滑表型，它会诱导产生针对脂多糖（LPS）的 O-多糖（O-PS）的抗体，因此无法区分接种疫苗的动物和感染动物。据推测，替代疫苗可能是缺乏 O-PS 的减毒活布鲁氏菌粗糙菌株。布鲁氏菌 melitensis B115 是一种天然减毒的粗糙菌株。O-PS 不暴露于表面，而是存在于细胞质中。我们将 B115 与 Rev1 进行比较，测试了 B115 对小鼠中布鲁氏菌 melitensis 和布鲁氏菌 ovis 感染的保护活性。还评估了接种 B115 后的残余毒力和体液反应。接种 B115 可对布鲁氏菌 melitensis 16M 和布鲁氏菌 ovis 攻击菌株产生显著的保护性免疫，与 Rev1 提供的保护相当。未检测到针对 O-PS 的干扰抗体，而通过基于 B115 的特异性补体结合试验监测 B115 疫苗接种情况。这些有前景的特征表明，应进一步评估布鲁氏菌 melitensis B115 作为目标动物宿主的疫苗。
