Department of Bacteriology, Kimron Veterinary Institute, Bet Dagan 50250, Israel.
Genomic Bioinformatics Laboratory, Department of Molecular Biology, Ariel University, Ariel 40700, Israel.
Int J Mol Sci. 2020 Dec 8;21(24):9341. doi: 10.3390/ijms21249341.
species are facultative intracellular bacteria that cause brucellosis, a zoonotic world-wide disease. The live attenuated Rev.1 vaccine strain is widely used for the control of brucellosis in the small ruminant population. However, Rev.1 induces antibodies against the O-polysaccharide (O-PS) of the smooth lipopolysaccharide thus, it is difficult to differentiate between infected and vaccinated animals. Hence, rough strains lacking the O-PS have been introduced. In the current study, we conducted a comprehensive comparative analysis of the genome sequence of two natural Rev.1 rough strains, isolated from sheep, against that of 24 Rev.1 smooth strains and the virulent reference strain 16M. We identified and characterized eight vital mutations within highly important genes associated with lipopolysaccharide (LPS) biosynthesis and virulence, which may explain the mechanisms underlying the formation of the Rev.1 rough phenotype and may be used to determine the mechanism underlying virulence attenuation. Further complementation studies aimed to estimate the specific role of these mutations in affecting morphology and virulence will serve as a basis for the design of new attenuated vaccines for animal immunization against brucellosis.
绵羊附睾种布鲁氏菌是兼性细胞内细菌，可引起布鲁氏菌病，这是一种在全世界流行的动物传染病。活的减毒 Rev.1 疫苗株被广泛用于控制小反刍动物群中的布鲁氏菌病。然而，Rev.1 诱导针对光滑脂多糖的 O-多糖（O-PS）的抗体，因此，很难区分感染动物和接种疫苗的动物。因此，已经引入了缺乏 O-PS 的粗糙菌株。在本研究中，我们对从绵羊中分离的两个天然 Rev.1 粗糙菌株的基因组序列与 24 个 Rev.1 光滑菌株和毒力参考菌株 16M 的基因组序列进行了全面比较分析。我们确定并表征了与脂多糖（LPS）生物合成和毒力相关的高度重要基因中的八个重要突变，这些突变可能解释了 Rev.1 粗糙表型形成的机制，并可能用于确定毒力衰减的机制。进一步的互补研究旨在估计这些突变在影响形态和毒力方面的特定作用，这将为设计针对动物布鲁氏菌病免疫接种的新型减毒疫苗提供基础。
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