González N V, Soloneski S, Larramendy M L
Cátedra de Citología, Facultad de Ciencias Naturales y Museo, Universidad Nacional de La Plata, La Plata, Argentina.
J Hazard Mater. 2009 Apr 15;163(1):337-43. doi: 10.1016/j.jhazmat.2008.06.097. Epub 2008 Jul 3.
In the present study the cytogenetic and genotoxic effect of benzoic herbicide dicamba and its Argentinean commercial formulation banvel (57.71% dicamba) was evaluated and whether this effect is mediated through oxidative damage or not. The protective role of vitamin E was also studied. Sister chromatid exchange (SCE) frequency, cell-cycle progression, and cell viability analyses in CHO cells were used as in vitro end-points. Treatments with the test compounds were performed either during 24h (Protocol A) or 12h (Protocol B) before harvesting. Protocol A showed that vitamin E decreased pesticide SCE induction, corrected the cell-cycle delay and partially protected cell-death only in 500 microg/ml dicamba-treated cultures. A similar trend was found in banvel-treated cultures. Protocol B revealed similar protective role of vitamin E only for dicamba-induced geno- and cytotoxicity. Based on these observations it could be suggested that dicamba injures DNA by delivering reactive oxygen species rather than by another type of mechanism/s. Although banvel mimics the effect observed by dicamba, its formulation contains other xenobiotic/s agents able to induce cellular and DNA damage by a different mechanism/s. Further investigations are needed to acquire a comprehensive knowledge of the possible mechanism/s through dicamba and banvel exert their toxic effects.
在本研究中，评估了苯甲酸类除草剂麦草畏及其阿根廷商业配方百草敌（57.71%麦草畏）的细胞遗传学和基因毒性作用，以及这种作用是否通过氧化损伤介导。还研究了维生素E的保护作用。将CHO细胞中的姐妹染色单体交换（SCE）频率、细胞周期进程和细胞活力分析用作体外终点指标。在收获前，用受试化合物进行24小时（方案A）或12小时（方案B）的处理。方案A表明，维生素E仅在500微克/毫升麦草畏处理的培养物中降低了农药诱导的SCE，纠正了细胞周期延迟并部分保护了细胞死亡。在百草敌处理的培养物中发现了类似的趋势。方案B显示，维生素E仅对麦草畏诱导的基因毒性和细胞毒性具有类似的保护作用。基于这些观察结果，可以认为麦草畏通过传递活性氧而非通过其他类型的机制损伤DNA。尽管百草敌模拟了麦草畏观察到的效果，但其配方含有其他能够通过不同机制诱导细胞和DNA损伤的外源性物质。需要进一步研究以全面了解麦草畏和百草敌发挥其毒性作用的可能机制。
