Faculty of Food Technology and Biotechnology, University of Zagreb, Zagreb, Croatia.
Toxicol Mech Methods. 2009 Oct;19(8):518-23. doi: 10.1080/15376510903280107.
Lindane, a toxic insecticide from the persistent organic pollutants (POP's) group, may act as an endocrine disrupter affecting crucial tissues of reproductive system. In this study a Chinese Hamster Ovary cell line (CHO-K1) was applied to assess the potential of lindane cytotoxicity at the cellular level. The methods of Trypan blue exclusion, MTT and Kenacid blue assays were used to assess cytotoxicity and confirmed a decrease in the number of viable CHO-K1 cells at 34.4-344 microM lindane during 24, 48 and 72 hours of exposure. The cell proliferation tests showed significant inhibition (p < 0.025-0.001 vs control) and a progressive increase in toxicity with increasing lindane concentrations. Corresponding IC(50) values were determined with each applied method. After 72 h of lindane exposure, IC(50) values were 184 microM according to the Trypan blue method and 272 and 256 microM with the Kenacid blue and MTT assays, respectively. Morphological changes induced by the cytotoxicity of lindane were followed by the fluorescence microscopy and only necrotic cells were detected. Vitamin E (25 and 50 microg/mL) was used for protection of ovarian cells against lidane-induced oxidative stress damage, and lipid peroxidation was postulated as a possible mechanism of lindane toxicity. The viability of cells pre-incubated with vitamin E was significantly enhanced (up to p < 0.025) compared to the results observed in cells exposed to lindane only, but vitamin E treatment could not prevent complete lindane-induced cytotoxicity. Results suggest that vitamin E may exert a slightly protective role in cell defense against lipophilic pro-oxidant xenobiotics such as lindane.
林丹，一种持久性有机污染物（POP）类有毒杀虫剂，可能作为一种内分泌干扰物，影响生殖系统的关键组织。在这项研究中，应用中国仓鼠卵巢细胞系（CHO-K1）来评估林丹细胞毒性在细胞水平的潜在作用。采用台盼蓝排斥、MTT 和肯那蓝检测法评估细胞毒性，并证实林丹暴露 24、48 和 72 小时后，在 34.4-344μM 浓度范围内，CHO-K1 细胞数量减少。细胞增殖试验表明，林丹浓度增加导致显著抑制（p<0.025-0.001 与对照组相比）和毒性增加。用每种应用方法确定相应的 IC50 值。林丹暴露 72 小时后，根据台盼蓝法 IC50 值为 184μM，肯那蓝和 MTT 法分别为 272 和 256μM。林丹细胞毒性引起的形态学变化通过荧光显微镜观察，仅检测到坏死细胞。维生素 E（25 和 50μg/mL）用于保护卵巢细胞免受林丹诱导的氧化应激损伤，并且脂质过氧化作用被认为是林丹毒性的可能机制。用维生素 E 预先孵育的细胞活力明显增强（高达 p<0.025），与仅暴露于林丹的细胞观察到的结果相比，但维生素 E 处理不能防止完全的林丹诱导的细胞毒性。结果表明，维生素 E 可能在细胞防御亲脂性促氧化剂等外源化学物质方面发挥轻微的保护作用，如林丹。
