一株能够感染2型猪链球菌菌株的噬菌体的分离与鉴定

Isolation and identification of a bacteriophage capable of infecting Streptococcus suis type 2 strains.

作者信息

Ma Y L, Lu C P

机构信息

Key Lab Animal Disease Diagnostic & Immunology, Ministry of Agriculture, Nanjing Agricultural University, Nanjing 210095, PR China.

出版信息

Vet Microbiol. 2008 Dec 10;132(3-4):340-7. doi: 10.1016/j.vetmic.2008.05.013. Epub 2008 May 18.

DOI:10.1016/j.vetmic.2008.05.013

PMID:18676101

Abstract

Streptococcus suis (S. suis) type 2 infection is considered to be a major problem worldwide in the swine industry. Studying phages of S. suis type 2 would be crucial for understanding the ecology and evolution of the Gram-positive bacteria. However, at the present, very little is known about them. An S. suis type 2 bacteriophage, named SMP, was isolated from nasal swabs of healthy Bama minipigs and was characterized at the microbiological and molecular levels. Phage SMP had an isometric head of 50 nm, a noncontractile tail of approximately 135 nm, and a linear double-stranded DNA genome. The host range of phage SMP was limited to 2 of 24 S. suis type 2 strains tested. The genome of phage SMP contained 36,216 bp with an average G+C content of 41.6%.

摘要

猪链球菌2型感染被认为是全球养猪业的一个主要问题。研究猪链球菌2型噬菌体对于理解这种革兰氏阳性菌的生态学和进化至关重要。然而，目前对它们的了解非常少。从健康巴马香猪的鼻拭子中分离出一种猪链球菌2型噬菌体，命名为SMP，并在微生物学和分子水平上对其进行了表征。噬菌体SMP有一个50纳米的等轴头、一条约135纳米的非收缩性尾和一个线性双链DNA基因组。噬菌体SMP的宿主范围仅限于所测试的24株猪链球菌2型菌株中的2株。噬菌体SMP的基因组包含36216个碱基对，平均G+C含量为41.6%。