Kim Hyun Jik, Lee Jeung-Gweon, Kang Ju Wan, Cho Hyung-Ju, Kim Hyun Su, Byeon Hyung Kwon, Yoon Joo-Heon
Department of Medicine Graduate School, Yonsei University College of Medicine, Seoul, Korea.
Laryngoscope. 2008 Oct;118(10):1862-7. doi: 10.1097/MLG.0b013e31817f4d34.
OBJECTIVES/HYPOTHESIS: Saline irrigation would be more effective for chronic sinusitis patients if it had bactericidal effects. Low concentrations of hypochlorous acid may be used as a nasal irrigation solution. First, we developed a 0.85% NaCl solution by adding NaCl to tap water (pH 7.0 and 8.4) and measuring the concentration of free chlorine and hypochlorous acid after giving the solution a short electrical impulse of 20 seconds. Then we investigated whether the derived hypochlorous acid had a toxic effect on human primary nasal epithelial cells, if and what effect it had on the expression of mucin genes, and, finally, if it had bactericidal, fungicidal, or virucidal effects.
In vitro biochemical experiment.
We treated human primary nasal epithelial cells with 3.5 ppm of hypochlorous acid and then examined the cells for cytotoxicity. We also investigated the bactericidal, fungicidal, and virucidal effects by challenging the cells with the following microorganisms Aspergillus fumigatus, Haemophilus influenzae, Klebsiella pneumoniae, Rhizopus oryzae, Candida albicans, Methicillin-resistant Staphylococcus aureus, Streptococcus pneumoniae, and Streptococcus pyogenes. To study the virucidal effects of HOCl, we used the human influenza A virus to challenge the cells.
: In the cytotoxicity assay and in the morphological examination, the cells did not show anytoxicity at 30 minute or 2 hours after treatment with HOCl. More than 99% of bactericidal or fungicidal activity was noted for all species, except for Candida albicans, in tap water at either pH 7.0 or 8.4. In addition, a 3.2-log10 reduction was achieved in cells challenged with the human influenza A virus.
A low concentration HOCl solution can be used as an effective nasal irrigation solution.
目的/假设：如果盐水冲洗具有杀菌作用，那么它对慢性鼻窦炎患者可能会更有效。低浓度的次氯酸可用作鼻腔冲洗液。首先，我们通过向自来水中添加氯化钠（pH值为7.0和8.4），并在给予该溶液20秒的短电脉冲后测量游离氯和次氯酸的浓度，从而配制出0.85%的氯化钠溶液。然后，我们研究了所产生的次氯酸对人原代鼻上皮细胞是否具有毒性作用，对粘蛋白基因表达是否有影响，以及最终是否具有杀菌、杀真菌或杀病毒作用。
体外生化实验。
我们用3.5 ppm的次氯酸处理人原代鼻上皮细胞，然后检测细胞的细胞毒性。我们还通过用以下微生物攻击细胞来研究杀菌、杀真菌和杀病毒作用：烟曲霉、流感嗜血杆菌、肺炎克雷伯菌、米根霉、白色念珠菌、耐甲氧西林金黄色葡萄球菌、肺炎链球菌和化脓性链球菌。为了研究次氯酸的杀病毒作用，我们用人甲型流感病毒攻击细胞。
在细胞毒性试验和形态学检查中，用次氯酸处理30分钟或2小时后，细胞未显示出任何毒性。在pH值为7.0或8.4的自来水中，除白色念珠菌外，所有物种的杀菌或杀真菌活性均超过99%。此外，用人甲型流感病毒攻击的细胞实现了3.2个对数级的减少。
低浓度的次氯酸溶液可用作有效的鼻腔冲洗液。
