幽门螺杆菌NADP依赖型3-磷酸甘油醛脱氢酶的表达、纯化、结晶及初步X射线分析
Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.
作者信息
Elliott Paul R, Evans Daniel, Greenwood Jacqueline A, Moody Peter C E
机构信息
Henry Wellcome Laboratories for Structural Biology, Department of Biochemistry, University of Leicester, Leicester LE1 9HN, England.
出版信息
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):723-6. doi: 10.1107/S1744309108020320. Epub 2008 Jul 26.
Abstract
The classical glycolytic pathway contains an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, with NADP-dependent forms reserved for photosynthetic organisms and archaea. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori is reported; crystals of the protein were grown both in the presence and the absence of NADP.
摘要
经典糖酵解途径包含一种依赖NAD的3-磷酸甘油醛脱氢酶，而依赖NADP的形式则存在于光合生物和古细菌中。本文报道了幽门螺杆菌中一种依赖NADP的3-磷酸甘油醛脱氢酶的克隆、表达、纯化、结晶及初步X射线分析；该蛋白晶体在有和没有NADP的情况下均已生长出来。
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引用本文的文献
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Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.幽门螺杆菌NAD依赖型3-磷酸甘油醛脱氢酶的表达、纯化、结晶及X射线初步分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):727-9. doi: 10.1107/S1744309108020368. Epub 2008 Jul 26.
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