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幽门螺杆菌NADP依赖型3-磷酸甘油醛脱氢酶的表达、纯化、结晶及初步X射线分析

Expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.

作者信息

Elliott Paul R, Evans Daniel, Greenwood Jacqueline A, Moody Peter C E

机构信息

Henry Wellcome Laboratories for Structural Biology, Department of Biochemistry, University of Leicester, Leicester LE1 9HN, England.

出版信息

Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):723-6. doi: 10.1107/S1744309108020320. Epub 2008 Jul 26.

DOI:10.1107/S1744309108020320
PMID:18678941
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC2494975/
Abstract

The classical glycolytic pathway contains an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase, with NADP-dependent forms reserved for photosynthetic organisms and archaea. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of an NADP-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori is reported; crystals of the protein were grown both in the presence and the absence of NADP.

摘要

经典糖酵解途径包含一种依赖NAD的3-磷酸甘油醛脱氢酶，而依赖NADP的形式则存在于光合生物和古细菌中。本文报道了幽门螺杆菌中一种依赖NADP的3-磷酸甘油醛脱氢酶的克隆、表达、纯化、结晶及初步X射线分析；该蛋白晶体在有和没有NADP的情况下均已生长出来。

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引用本文的文献

1
Expression, purification, crystallization and preliminary X-ray analysis of an NAD-dependent glyceraldehyde-3-phosphate dehydrogenase from Helicobacter pylori.幽门螺杆菌NAD依赖型3-磷酸甘油醛脱氢酶的表达、纯化、结晶及X射线初步分析
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2008 Aug 1;64(Pt 8):727-9. doi: 10.1107/S1744309108020368. Epub 2008 Jul 26.

本文引用的文献

1
Scaling and assessment of data quality.数据质量的评估与分级
Acta Crystallogr D Biol Crystallogr. 2006 Jan;62(Pt 1):72-82. doi: 10.1107/S0907444905036693. Epub 2005 Dec 14.
2
The Entner-Doudoroff pathway has little effect on Helicobacter pylori colonization of mice.恩特纳-杜德洛夫途径对幽门螺杆菌在小鼠体内的定殖影响很小。
Infect Immun. 2003 May;71(5):2920-3. doi: 10.1128/IAI.71.5.2920-2923.2003.
3
Pushing the boundaries of molecular replacement with maximum likelihood.用最大似然法突破分子置换的界限。
Acta Crystallogr D Biol Crystallogr. 2001 Oct;57(Pt 10):1373-82. doi: 10.1107/s0907444901012471. Epub 2001 Sep 21.
4
The disease spectrum of Helicobacter pylori: the immunopathogenesis of gastroduodenal ulcer and gastric cancer.幽门螺杆菌的疾病谱：胃十二指肠溃疡和胃癌的免疫发病机制。
Annu Rev Microbiol. 2000;54:615-40. doi: 10.1146/annurev.micro.54.1.615.
5
Review article: exploring the link between Helicobacter pylori and gastric cancer.综述文章：探索幽门螺杆菌与胃癌之间的联系
Aliment Pharmacol Ther. 1999 Mar;13 Suppl 1:3-11. doi: 10.1046/j.1365-2036.1999.00002.x.
6
Helicobacter pylori.幽门螺杆菌
Clin Microbiol Rev. 1997 Oct;10(4):720-41. doi: 10.1128/CMR.10.4.720.
7
The prevalence of Helicobacter pylori in peptic ulcer disease.幽门螺杆菌在消化性溃疡疾病中的患病率。
Aliment Pharmacol Ther. 1995;9 Suppl 2:59-69.
8
Glucose phosphorylation in Helicobacter pylori.幽门螺杆菌中的葡萄糖磷酸化作用
Arch Biochem Biophys. 1993 Jan;300(1):522-5. doi: 10.1006/abbi.1993.1071.
9
Glucose utilization and lactate production by Helicobacter pylori.幽门螺杆菌对葡萄糖的利用及乳酸生成
J Gen Microbiol. 1993 Dec;139(12):3023-8. doi: 10.1099/00221287-139-12-3023.
10
The Entner-Doudoroff pathway in Helicobacter pylori.幽门螺杆菌中的恩特纳-杜德洛夫途径。
Arch Biochem Biophys. 1994 Aug 1;312(2):349-56. doi: 10.1006/abbi.1994.1319.