Tourigny David S, Elliott Paul R, Edgell Louise J, Hudson Gregg M, Moody Peter C E
Henry Wellcome Laboratories for Structural Biology, Henry Wellcome Building, University of Leicester, Leicester LE1 9HN, England.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2011 Jan 1;67(Pt 1):72-5. doi: 10.1107/S1744309110044465. Epub 2010 Dec 22.
The genome of the enteric pathogen Campylobacter jejuni encodes a single glyceraldehyde-3-phosphate dehydrogenase that can utilize either NADP+ or NAD+ as coenzymes for the oxidative phosphorylation of glyceraldehyde-3-phosphate to 1,3-diphosphoglycerate. Here, the cloning, expression, purification, crystallization and preliminary X-ray analysis of both the wild type and an active-site mutant of the enzyme are presented. Preliminary X-ray analysis revealed that in both cases the crystals diffracted to beyond 1.9 Å resolution. The space group is shown to be I4(1)22, with unit-cell parameters a=90.75, b=90.75, c=225.48 Å, α=90.46, β=90.46, γ=222.79°; each asymmetric unit contains only one subunit of the tetrameric enzyme.
肠道病原体空肠弯曲菌的基因组编码一种单一的甘油醛-3-磷酸脱氢酶，该酶可利用NADP⁺或NAD⁺作为辅酶，将甘油醛-3-磷酸氧化磷酸化为1,3-二磷酸甘油酸。本文介绍了该酶野生型和活性位点突变体的克隆、表达、纯化、结晶及初步X射线分析。初步X射线分析表明，在这两种情况下，晶体衍射分辨率均超过1.9 Å。空间群为I4(1)22，晶胞参数a=90.75、b=90.75、c=225.48 Å，α=90.46、β=90.46、γ=222.79°；每个不对称单元仅包含四聚体酶的一个亚基。
