Wu Katherine Lily, Berger Trish
Department of Animal Science, University of California, Davis, CA 95616, USA.
Bull Environ Contam Toxicol. 2008 Nov;81(5):490-3. doi: 10.1007/s00128-008-9509-3. Epub 2008 Aug 5.
Trichloroethylene (TCE), a commonly used industrial degreasing solvent and environmental toxicant, reduces rat oocyte fertilizability by an incompletely understood mechanism. Previous evidence implicated cytochrome P450 dependent oxidation of TCE. The current study investigated a second pathway, glutathione conjugation using S-(1,2-dichlorovinyl)-L-cysteine (DCVC), a mutagenic and cytotoxic TCE-metabolite. In vitro exposure of oocytes and in vivo exposure of females to DCVC significantly reduced oocyte fertilizability (63% vs. 26%; p < 0.005 and 60% vs. 36%; p < 0.005, respectively). Reduced fertilizability of oocytes following in vivo TCE exposure may be mediated partially by the glutathione conjugation pathway.
三氯乙烯（TCE）是一种常用的工业脱脂溶剂和环境毒物，其通过一种尚未完全明确的机制降低大鼠卵母细胞的受精能力。先前的证据表明TCE的细胞色素P450依赖性氧化作用与之相关。当前的研究调查了第二条途径，即使用S-（1,2-二氯乙烯基）-L-半胱氨酸（DCVC）进行谷胱甘肽结合反应，DCVC是一种具有致突变性和细胞毒性的TCE代谢产物。卵母细胞的体外暴露以及雌性动物的体内暴露于DCVC均显著降低了卵母细胞的受精能力（分别为63%对26%；p<0.005以及60%对36%；p<0.005）。体内TCE暴露后卵母细胞受精能力的降低可能部分由谷胱甘肽结合途径介导。
