Sridevi N, Prabhune A A
Biochemical Sciences Division, National Chemical Laboratory, Pune 411008, India.
Appl Biochem Biotechnol. 2009 May;157(2):254-62. doi: 10.1007/s12010-008-8326-9. Epub 2008 Aug 5.
A thermophilic microorganism growing within the temperature range of 40-65 degrees C (optimum at 55 degrees C) was isolated from hot water springs near Konkan, Maharashtra, India. Based on 16S rDNA sequence analysis, it was concluded that the isolate belongs to the genus Brevibacillus. The present paper reports the isolation, identification, and standardization of fermentation conditions for the production of enzyme, bile salt hydrolase (EC 3.5.1.24) which is produced intracellularly at high temperatures. This is the first report regarding the production of bile salt hydrolase from a thermophilic source. Optimization of fermentation conditions resulted in a 2.9-fold enhancement in enzyme production.
从印度马哈拉施特拉邦孔坎附近的温泉中分离出一种嗜热微生物，其生长温度范围为40-65摄氏度（最适温度为55摄氏度）。基于16S rDNA序列分析，得出该分离株属于短芽孢杆菌属的结论。本文报道了高温下胞内产生的酶——胆汁盐水解酶（EC 3.5.1.24）的分离、鉴定及发酵条件的标准化。这是关于从嗜热来源生产胆汁盐水解酶的首次报道。发酵条件的优化使酶产量提高了2.9倍。
