Thakur M K, Ghosh Swati
Department of Zoology, Banaras Hindu University, Varanasi, India.
J Mol Neurosci. 2009 Mar;37(3):269-73. doi: 10.1007/s12031-008-9131-1. Epub 2008 Aug 5.
We have reported earlier that estrogen receptor (ER) alpha-transactivation domain (TAD) interacted with four nuclear proteins of 100 kD, 80 kD, 68 kD, and 50 kD of mouse brain and identified 68 kD as p68 RNA helicase and 50 kD as beta-tubulin. In this paper, we describe the identification of 80 kD nuclear protein as metastasis associated protein 1 (MTA1) and its interaction and expression in the brain of aging mice. Far-Western blotting and immunoprecipitation data revealed lower interaction of MTA1 in old than adult mice of both sexes. Furthermore, adult male showed lower expression of protein as compared to adult female. Altogether these findings suggest that age-dependent decrease in the expression of MTA1 and its interaction with ERalpha-TAD may influence the estrogen-mediated signaling pathway during aging of mouse brain.
我们之前报道过，雌激素受体（ER）α反式激活结构域（TAD）与小鼠脑内100 kD、80 kD、68 kD和50 kD的四种核蛋白相互作用，并鉴定出68 kD的蛋白为p68 RNA解旋酶，50 kD的蛋白为β微管蛋白。在本文中，我们描述了将80 kD核蛋白鉴定为转移相关蛋白1（MTA1）及其在衰老小鼠脑内的相互作用和表达情况。Far-Western印迹法和免疫沉淀数据显示，老年小鼠中MTA1的相互作用低于成年雌雄小鼠。此外，成年雄性小鼠的蛋白表达低于成年雌性小鼠。总之，这些发现表明，MTA1表达的年龄依赖性降低及其与ERα-TAD的相互作用可能会影响小鼠脑衰老过程中雌激素介导的信号通路。
