[广州4194份脐血样本的HLA - A、B、DRB1等位基因及单倍型频率研究]

[Study of the HLA-A, B, DRB1 allele and haplotype frequencies of 4194 cord blood samples in Guangzhou].

作者信息

Sha Wen-qiong, Liao Can, Li Yan, Yang Xin, Tang Xue-wei, Huang Yi-ning, Xie Xing-mei

机构信息

Guangzhou Maternal and Fetal Hospital, Guangzhou Medical College, Guangzhou, Guangdong, 510180 People's Republic of China.

出版信息

Zhonghua Yi Xue Yi Chuan Xue Za Zhi. 2008 Aug;25(4):473-6.

PMID:18683155

Abstract

OBJECTIVE

To analyze the distribution of the human leukocyte antigen (HLA)-A, B and DRB1 allele and haplotype in cord blood samples preserved in Guangzhou Cord Blood Bank collected in the last 10 years.

METHODS

The HLA-A, B and DRB1 genotyping of 4194 cord blood samples were detected by Special Monoclonal Tray, PCR-sequence specific promer (PCR-SSP), PCR-sequence specific oligonucleotide probe (PCR-SSO) and sequence based typing (SBT). Frequencies of HLA-A, B and DRB1 allele and haplotype were calculated by Arlequin software.

RESULTS

The total numbers of HLA-A, B and DRB1 alleles are 18, 43, 13 respectively. The obviously high frequency alleles are A11, A02, A24, A33, B40, B15, B46, B13, DRB112, DRB115, DRB109 and DRB104, with accumulative frequency of each locus being more than 50%. The most common haplotypes are A2-B46, B46-DR9, A11-DR12 and A2-B46-DR9.

CONCLUSION

The distribution of HLA-A, B and DRB1 allele and haplotype of cord blood in Guangzhou Cord Blood Bank has typical characteristics of southern Chinese Han population. Authors' data may help in searching for appropriate donors.

摘要

目的

分析广州脐血库近10年所保存脐血样本中人类白细胞抗原（HLA）-A、B及DRB1等位基因和单倍型的分布情况。

方法

采用特制单克隆板、聚合酶链反应-序列特异性引物（PCR-SSP）、聚合酶链反应-序列特异性寡核苷酸探针（PCR-SSO）及序列分型（SBT）技术对4194份脐血样本进行HLA-A、B及DRB1基因分型。利用Arlequin软件计算HLA-A、B及DRB1等位基因和单倍型的频率。

结果

HLA-A、B及DRB1等位基因总数分别为18个、43个、13个。明显高频等位基因为A11、A02、A24、A33、B40、B15、B46、B13、DRB112、DRB115、DRB109及DRB104，各基因座累计频率均超过50%。最常见的单倍型为A2-B46、B46-DR9、A11-DR12及A2-B46-DR9。