Booth P L, Thomas W E
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
Brain Res. 1991 May 10;548(1-2):163-71. doi: 10.1016/0006-8993(91)91118-k.
Ramified microglial cells were investigated in primary cultures of dissociated cerebral cortical tissue from rats. The identification of these cells was confirmed through immunohistochemical staining with 7 monoclonal antibodies selective for microglia. While there was significant variation in staining intensity with different antibodies, all stained the identified ramified cells; the antibodies OX-42 and ED1 yielded the most intense immunoreactivity. Based on distinctive morphological features, the microglia could be identified in living cultures where they were monitored using time-lapse video recording. This technique revealed extremely dynamic features of cellular plasticity and motility. Ramified microglia exhibited constant and rapid alterations in the size and shape of their cell body with an associated extension and retraction of processes; concomitantly, the cells moved about in a circumscribed area. These features of plasticity and motility were unique to this cell type, and correlated with OX-42 immunostaining. The microglia also possessed a differentially high level of pinocytotic activity; this too was correlated with OX-42 staining. From the nature of their morphological plasticity and motility, high pinocytosis, and cellular distribution, it is hypothesized that the ramified microglia specifically function as a system of fluid cleansing in normal brain tissue.
在大鼠大脑皮质组织解离的原代培养物中对分支状小胶质细胞进行了研究。通过用7种对小胶质细胞具有选择性的单克隆抗体进行免疫组织化学染色，证实了这些细胞的身份。虽然不同抗体的染色强度存在显著差异，但所有抗体都对鉴定出的分支状细胞进行了染色；抗体OX - 42和ED1产生的免疫反应最强。基于独特的形态特征，可以在活细胞培养物中识别小胶质细胞，并使用延时视频记录对其进行监测。这项技术揭示了细胞可塑性和运动性的极其动态的特征。分支状小胶质细胞的细胞体大小和形状不断快速变化，同时其突起会伸长和缩回；与此同时，细胞在一个限定区域内移动。这些可塑性和运动性特征是这种细胞类型所特有的，并且与OX - 42免疫染色相关。小胶质细胞还具有不同程度的高吞噬活性；这也与OX - 42染色相关。从其形态可塑性和运动性、高吞噬作用以及细胞分布的性质来看，推测分支状小胶质细胞在正常脑组织中专门起到液体清除系统的作用。
