Ranson P A, Thomas W E
Department of Oral Biology, College of Dentistry, Ohio State University, Columbus 43210.
J Histochem Cytochem. 1991 Jun;39(6):853-8. doi: 10.1177/39.6.2033242.
Based on previous observations in tissue culture, we investigated pinocytotic activity as a potential cell marker for brain microglia. This functional activity was assessed in three different preparations derived from rat: primary cultures of mixed cerebral cortical cells, tissue slabs of whole cerebrum, and cultures of isolated or enriched microglial cells. Each preparation was incubated with the fluorescent dye lucifer yellow as a soluble tracer and then processed for light microscopy. Under the conditions utilized, ramified microglia specifically exhibited differentially high pinocytotic labeling in all cases; the dye was mainly localized within the cell somata, where it was sequestered in pinocytotic vesicles. In each preparation, the identity of the labeled cell population was confirmed as microglia through immunohistochemical staining with the monoclonal antibody (MAb) OX-42, a specific microglial marker. Therefore, pinocytotic labeling is proposed as a select cell marker for microglia, which may be extremely useful in the identification and study of ramified microglial cells.
基于先前在组织培养中的观察结果，我们研究了胞饮活性作为脑小胶质细胞潜在细胞标志物的情况。在源自大鼠的三种不同制剂中评估了这种功能活性：混合大脑皮质细胞的原代培养物、全脑的组织切片以及分离或富集的小胶质细胞培养物。每种制剂都与荧光染料路西法黄作为可溶性示踪剂一起孵育，然后进行光学显微镜检查。在所采用的条件下，分支状小胶质细胞在所有情况下均特异性地表现出不同程度的高胞饮标记；染料主要定位于细胞体中，并被隔离在胞饮小泡中。在每种制剂中，通过使用特异性小胶质细胞标志物单克隆抗体（MAb）OX-42进行免疫组织化学染色，确认标记细胞群体为小胶质细胞。因此，胞饮标记被提议作为小胶质细胞的一种选择性细胞标志物，这在分支状小胶质细胞的鉴定和研究中可能极其有用。
