Jordan F L, Thomas W E
Department of Physiology, Meharry Medical College, Nashville, TN 37208.
Brain Res Bull. 1987 Jul;19(1):153-9. doi: 10.1016/0361-9230(87)90180-8.
Microglia were investigated in tissue cultures of dissociated cerebral cortex from embryonic rat. Three different forms of microglia were identified in relatively mature cultures on the basis of morphological criteria, esterase histochemistry, and immunohistochemical staining for Fc receptors. Two of these forms corresponded to the amoeboid and ramified microglia; the amoeboid form stained intensely for esterase activity and ramified cells possessed Fc receptors. The third microglial cell form was distinguished by its morphological properties and was characterized by the presence of numerous filopodial processes. This morphological form occasionally possessed esterase staining and was the predominate type of microglia observed in the cultures. The identification of these cell types supports the potential use of this culture system to investigate functional aspects of microglia and their interaction with other brain cells.
在来自胚胎大鼠的解离大脑皮质组织培养物中对小胶质细胞进行了研究。基于形态学标准、酯酶组织化学以及Fc受体的免疫组织化学染色，在相对成熟的培养物中鉴定出了三种不同形式的小胶质细胞。其中两种形式分别对应于阿米巴样小胶质细胞和分支状小胶质细胞；阿米巴样形式的细胞酯酶活性染色强烈，而分支状细胞具有Fc受体。第三种小胶质细胞形式以其形态学特性而与众不同，其特征是存在大量丝状伪足突起。这种形态形式偶尔具有酯酶染色，并且是培养物中观察到的主要小胶质细胞类型。这些细胞类型的鉴定支持了该培养系统在研究小胶质细胞功能方面及其与其他脑细胞相互作用的潜在用途。
