培养的神经元和神经胶质细胞中的磷脂酶C同工酶：一项免疫细胞化学和免疫化学研究。

Phospholipase C isozymes in neurons and glial cells in culture: an immunocytochemical and immunochemical study.

作者信息

Mizuguchi M, Yamada M, Kim S U, Rhee S G

机构信息

Department of Medicine, University of British Columbia, Vancouver, Canada.

出版信息

Brain Res. 1991 May 10;548(1-2):35-40. doi: 10.1016/0006-8993(91)91103-8.

DOI:10.1016/0006-8993(91)91103-8

PMID:1868346

Abstract

Expression of 3 brain isozymes of phospholipase (PLC-beta, PLC-gamma and PLC-delta) was investigated in relation to cell types found in rat CNS cultures. Immunoreactivity of cultured neurons, astrocytes and oligodendrocytes was demonstrated for all of the 3 isozymes by immunocytochemical staining and immunoblotting, with some differences in reaction intensity. Immunoblotting revealed that the level of expression was neurons greater than oligodendrocytes greater than astrocytes for PLC-beta and PLC-gamma, and astrocytes greater than oligodendrocytes greater than neurons for PLC-delta.

摘要

研究了磷脂酶的3种脑同工酶（PLC-β、PLC-γ和PLC-δ）的表达与大鼠中枢神经系统培养物中发现的细胞类型的关系。通过免疫细胞化学染色和免疫印迹法证实，培养的神经元、星形胶质细胞和少突胶质细胞对所有3种同工酶均具有免疫反应性，但反应强度存在一些差异。免疫印迹显示，PLC-β和PLC-γ的表达水平为神经元大于少突胶质细胞大于星形胶质细胞，而PLC-δ的表达水平为星形胶质细胞大于少突胶质细胞大于神经元。