Dunn A J, Antoon M, Chapman Y
Department of Pharmacology and Therapeutics, Louisiana State University Medical Center, Shreveport 71130-3932.
Brain Res Bull. 1991 Apr;26(4):539-42. doi: 10.1016/0361-9230(91)90092-x.
The behavior of mice was scored in a multicompartment chamber one hour following intraperitoneal injection of recombinant human interleukin-1 (IL-1). Both IL-1 alpha and IL-1 beta dose-dependently reduced the mean duration for which mice were in contact with novel stimuli without altering measures of locomotor activity, such as movements between the compartments or rears. These behavioral changes resemble those previously observed with prior restraint or intracerebroventricular (ICV) injection of corticotropin-releasing factor (CRF). Effective doses were in the range 0.1-10 ng for IL-1 alpha, and 1-10 ng for IL-1 beta. The reduction in stimulus-contact times induced by 1 ng of IL-1 beta was reversed by prior ICV injection of the CRF antagonist, alpha-helical CRF9-41, suggesting that IL-1 causes secretion of brain CRF which in turn elicits the behavioral changes. These results indicate that peripheral administration of IL-1 alpha or IL-1 beta in low doses can alter behavior. They provide additional evidence that IL-1 administration stimulates brain CRF secretion, and that brain CRF can modulate exploratory behavior, and thus reinforces the concept that IL-1 administration can induce stress.
在腹腔注射重组人白细胞介素-1（IL-1）一小时后，在多隔室实验箱中对小鼠的行为进行评分。IL-1α和IL-1β均呈剂量依赖性地缩短了小鼠与新刺激接触的平均持续时间，而不改变运动活动的指标，如隔室间的移动或站立次数。这些行为变化类似于先前在预先约束或脑室内（ICV）注射促肾上腺皮质激素释放因子（CRF）时观察到的变化。IL-1α的有效剂量范围为0.1 - 10 ng，IL-1β的有效剂量范围为1 - 10 ng。预先脑室内注射CRF拮抗剂α-螺旋CRF9 - 41可逆转1 ng IL-1β诱导的刺激接触时间缩短，这表明IL-1会导致脑CRF分泌，进而引发行为变化。这些结果表明，外周给予低剂量的IL-1α或IL-1β可改变行为。它们提供了额外的证据，证明给予IL-1会刺激脑CRF分泌，并且脑CRF可调节探索行为，从而强化了给予IL-1可诱导应激的概念。
