Kassabov K T, Stoychkov J N
Medical Academy, Department of Experimental Cancer Therapy, Sofia, Bulgaria.
Cancer Immunol Immunother. 1991;33(5):307-13. doi: 10.1007/BF01756595.
The antimetastatic activity of orally administered polybacterial vaccines, Broncho-Vaxom (BV) and Respivax (RV) was examined in C57BL/6 mice, bearing implants of Lewis lung carcinoma (3LL) in the footpad. The oral administration of BV or RV for 10 consecutive days before or after surgery caused significant reduction of the number and volume of lung metastases. In addition, the therapeutic potential of BV and RV was examined in combination with chemotherapy to determine if there is additive activity. In animals bearing pulmonary micrometastases, treatment with a combination of cyclophosphamide at 50-150 mg/kg with BV or RV was found to be more effective than each of these treatments alone. In immune function studies it was established that the oral administration of BV and RV induced an increase in the number of cells, recovered by broncho-alveolar lavage, and alveolar macrophages were dominant in these cell populations. Furthermore, oral treatment of mice with these vaccines rendered their alveolar macrophages tumoricidal for syngeneic metastatic 3LL cells in vitro. These results show that pulmonary macrophages induced by oral administration of BV and RV played a key role in the inhibition of metastasis in 3LL-bearing mice.
在足垫植入Lewis肺癌（3LL）的C57BL/6小鼠中，检测了口服多细菌疫苗Broncho-Vaxom（BV）和Respivax（RV）的抗转移活性。在手术前或手术后连续10天口服BV或RV，可显著减少肺转移灶的数量和体积。此外，还检测了BV和RV与化疗联合使用的治疗潜力，以确定是否存在相加活性。在患有肺微转移的动物中，发现50-150mg/kg环磷酰胺与BV或RV联合治疗比单独使用这些治疗方法更有效。在免疫功能研究中确定，口服BV和RV可使支气管肺泡灌洗回收的细胞数量增加，且这些细胞群体中肺泡巨噬细胞占主导。此外，用这些疫苗对小鼠进行口服治疗，可使它们的肺泡巨噬细胞在体外对同基因转移性3LL细胞具有杀瘤作用。这些结果表明，口服BV和RV诱导的肺巨噬细胞在抑制荷3LL小鼠的转移中起关键作用。
