Talmadge J E, Schneider M, Collins M, Phillips H, Herberman R B, Wiltrout R H
J Immunol. 1985 Aug;135(2):1477-83.
Liposomes incorporating a variety of immunomodulators have been shown to activate macrophages and monocytes for tumoricidal activity both in vivo and in vitro. We report that in addition to the activation of macrophages, the i.v. injection of liposomes (multilamellar vesicles) that have encapsulated muramyl tripeptide-phosphatidylethanolamine (MTP-PE) could also augment interstitial natural killer (NK) cell activity in the lung and the liver. In contrast, liposomes incorporating MTP-PE were unable to augment NK cell activity in the spleen, peripheral blood, or peritoneal cavity (after i.p. injection). In addition, liposomes did not augment splenic NK cell activity in vitro. This suggests that the augmentation of NK cell activity in the lungs and liver was not due to direct effects of the liposomes but may have been a secondary effect mediated by a monokine. The augmentation of pulmonary NK cell activity was paralleled by the nonspecific immunoprophylaxis of experimental pulmonary metastases. The augmented NK cell activity, as well as the enhanced nonspecific immunoprophylactic activity, was reduced by pretreatment of the mice with anti-asialo GM1 antiserum. Thus, the augmentation of organ-associated NK cell activity by liposomes incorporating MTP/PE plays a major role in the host's increased resistance to the formation of experimental metastases.
已证明，包封了多种免疫调节剂的脂质体在体内和体外均可激活巨噬细胞和单核细胞，使其具有杀肿瘤活性。我们报告称，除了激活巨噬细胞外，静脉注射包封了胞壁酰三肽 - 磷脂酰乙醇胺（MTP - PE）的脂质体（多层囊泡）还可增强肺和肝脏中的间质自然杀伤（NK）细胞活性。相比之下，包封了MTP - PE的脂质体无法增强脾脏、外周血或腹腔（腹腔注射后）中的NK细胞活性。此外，脂质体在体外也不会增强脾脏NK细胞活性。这表明肺和肝脏中NK细胞活性的增强并非脂质体的直接作用，而可能是由一种单核因子介导的次级效应。肺NK细胞活性的增强与实验性肺转移的非特异性免疫预防作用平行。用抗唾液酸GM1抗血清预处理小鼠可降低增强的NK细胞活性以及增强的非特异性免疫预防活性。因此，包封了MTP/PE的脂质体对器官相关NK细胞活性的增强在宿主对实验性转移形成的抵抗力增加中起主要作用。
