Aligholi Marzieh, Emaneini Mohammad, Jabalameli Fereshteh, Shahsavan Shadi, Dabiri Hosein, Sedaght Hossein
Department of Microbiology, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Med Princ Pract. 2008;17(5):432-4. doi: 10.1159/000141513. Epub 2008 Aug 6.
The objective of the study was to investigate the prevalence of vancomycin-resistant Staphylococcus aureus (VRSA).
Three hundred and fifty-six S. aureus isolates from the Imam Khomeini hospital in Tehran, Iran, were evaluated for methicillin and decreased vancomycin susceptibility by the microbroth dilution method. The mecA, vanAand vanB genes were targeted by polymerase chain reaction.
Of the 356 isolates, 149 (41.85%) S. aureus strains were resistant to methicillin. Two strains of methicillin-resistant S. aureus were VRSA strains. One isolate, Teaching Hospital-1 (TEH-1), had a vancomycin minimum inhibitory concentration (MIC) of 64 microg/ml and was susceptible to teicoplanin while the other isolate (TEH-2) had a vancomycin and teicoplanin MIC of 512 and >256 microg/ml, respectively, and was positive for the vanA gene.
This report shows that the emergence of VRSA in Iran warrants active microbiological surveillance and careful monitoring of vancomycin therapy.
本研究的目的是调查耐万古霉素金黄色葡萄球菌（VRSA）的流行情况。
采用微量肉汤稀释法，对来自伊朗德黑兰伊玛目霍梅尼医院的356株金黄色葡萄球菌分离株进行甲氧西林耐药性及万古霉素敏感性降低情况评估。通过聚合酶链反应靶向检测mecA、vanA和vanB基因。
在356株分离株中，149株（41.85%）金黄色葡萄球菌菌株对甲氧西林耐药。两株耐甲氧西林金黄色葡萄球菌为VRSA菌株。一株分离株，教学医院1号（TEH-1），万古霉素最低抑菌浓度（MIC）为64μg/ml，对替考拉宁敏感，而另一株分离株（TEH-2）的万古霉素和替考拉宁MIC分别为512μg/ml和>256μg/ml，且vanA基因呈阳性。
本报告表明，伊朗VRSA的出现需要积极的微生物监测以及对万古霉素治疗的密切监测。
