Kirby Daniel J, Rosenkrands Ida, Agger Else M, Andersen Peter, Coombes Allan G A, Perrie Yvonne
Medicines Research Unit, School of Life and Health Sciences, Aston University, Birmingham, UK.
J Drug Target. 2008 Aug;16(7):543-54. doi: 10.1080/10611860802228558.
The ability of liposomes and microspheres to enhance the efficacy of a sub-unit antigen was investigated. Microspheres were optimised by testing a range of surfactants employed in the external aqueous phase of a water-in-oil-in-water (w/o/w) double emulsion solvent evaporation process for the preparation of microspheres--composed of poly(D,L-lactide-co-glycolide) and the immunological adjuvant dimethyl dioctadecyl ammonium bromide (DDA)--and then investigated with regard to the physico-chemical and immunological characteristics of the particles produced. The results demonstrate that this parameter can affect the physico-chemical characteristics of these systems and subsequently, has a substantial bearing on the level of immune response achieved, both humoral and cell mediated, when employed for the delivery of the sub-unit tuberculosis vaccine antigen Ag85B-ESAT-6. Moreover, the microsphere preparations investigated failed to initiate immune responses at the levels achieved with an adjuvant DDA-based liposome formulation (DDA-TDB), further substantiating the superior ability of liposomes as vaccine delivery systems.
研究了脂质体和微球增强亚单位抗原效力的能力。通过测试一系列用于水包油包水（w/o/w）双乳液溶剂蒸发法制备微球的外部水相中的表面活性剂来优化微球，该微球由聚（D，L-丙交酯-共-乙交酯）和免疫佐剂二甲基二十八烷基溴化铵（DDA）组成，然后研究了所产生颗粒的物理化学和免疫学特性。结果表明，该参数可影响这些系统的物理化学特性，随后，当用于递送亚单位结核疫苗抗原Ag85B-ESAT-6时，对所实现的体液和细胞介导的免疫反应水平有重大影响。此外，所研究的微球制剂未能引发与基于佐剂DDA的脂质体制剂（DDA-TDB）所达到的水平相当的免疫反应，进一步证实了脂质体作为疫苗递送系统的卓越能力。
