Kimber I, Quirke S, Cumberbatch M, Ashby J, Paton D, Aldridge R D, Hunter J A, Beck M H
ICI Central Toxicology Laboratory, Macclesfield, Cheshire, UK.
Contact Dermatitis. 1991 Mar;24(3):164-71. doi: 10.1111/j.1600-0536.1991.tb01692.x.
The use of a lymphocyte transformation test (LTT) to confirm allergic contact dermatitis from thiurams has been investigated. The responses of peripheral blood mononuclear cells (PBMC) from thiuram-sensitive and non-sensitive individuals following culture with dimethylcarbamoyl-protein (human serum albumin; HSA) and dimethylthiocarbamoyl-HSA conjugates has been compared. Only PBMC from those patients who were patch-test-positive with thiuram-mix and sensitized to tetramethylthiuram monosulphide (TMTM) or TMTM and tetramethylthiuram disulphide (TMTD) exhibited significant proliferative responses to these conjugates. Thiuram-patch-test-negative patients and control donors with no history of allergic contact dermatitis failed to mount a significant response to any concentration of either conjugate. Two of the thiuram-sensitive patients were also nickel-patch-test-positive, and PBMC isolated from these donors, but not from nickel-patch-test-negative patients, proved positive in a nickel LTT. The data reveal that relevant hapten-protein conjugates are capable of provoking specific human lymphocyte proliferative responses in vitro, and that, using this technique, the LTT can, in principle, be used for the investigation and/or diagnosis of skin sensitization to lipophilic contact allergens.
已对使用淋巴细胞转化试验（LTT）来确诊由秋兰姆引起的过敏性接触性皮炎进行了研究。比较了秋兰姆敏感和不敏感个体的外周血单个核细胞（PBMC）在用二甲基氨基甲酰蛋白（人血清白蛋白；HSA）和二甲基硫代氨基甲酰-HSA偶联物培养后的反应。只有那些对秋兰姆混合物斑贴试验呈阳性且对一硫化四甲基秋兰姆（TMTM）或TMTM和二硫化四甲基秋兰姆（TMTD）致敏的患者的PBMC对这些偶联物表现出显著的增殖反应。秋兰姆斑贴试验阴性的患者以及无过敏性接触性皮炎病史的对照供者对任何浓度的两种偶联物均未产生显著反应。两名秋兰姆敏感患者的镍斑贴试验也呈阳性，从这些供者分离的PBMC（而非镍斑贴试验阴性患者的PBMC）在镍LTT中呈阳性。数据表明，相关的半抗原-蛋白偶联物能够在体外激发特定的人类淋巴细胞增殖反应，并且使用这种技术，LTT原则上可用于研究和/或诊断对亲脂性接触变应原的皮肤致敏。
