Pascual I, de Miguel G Fernández, Gómez-Pinedo U A, de Miguel F, Arranz M García, García-Olmo D
Department of General Surgery, La Paz University Hospital, Madrid, Spain.
Br J Surg. 2008 Sep;95(9):1180-4. doi: 10.1002/bjs.6242.
The feasibility of producing 'biosutures' coated with adipose-derived mesenchymal stem cells (ASCs) was assessed and their effect on colonic anastomoses evaluated.
Sutures were cultured with ASCs isolated from BDIX rats before analysis by light and scanning electron microscopy. Anastomoses were performed in 40 BDIX rats using either biosutures or conventional sutures. Histopathological features, adhesion formation and anastomotic strength were evaluated.
Sutures were coated with ASCs within 24 h. Anastomoses made with biosutures had a lower adhesion index only during the first week (P = 0.006 at 7 days), but not subsequently. There were no significant differences in anastomotic healing with the two types of suture.
ASC biosutures have no effect on anastomotic healing in rats.
评估了生产涂有脂肪来源间充质干细胞（ASC）的“生物缝线”的可行性，并评估了其对结肠吻合术的影响。
在通过光学显微镜和扫描电子显微镜分析之前，将缝线与从BDIX大鼠分离的ASC一起培养。使用生物缝线或传统缝线对40只BDIX大鼠进行吻合术。评估组织病理学特征、粘连形成和吻合强度。
缝线在24小时内被ASC覆盖。使用生物缝线进行的吻合术仅在第一周具有较低的粘连指数（7天时P = 0.006），但随后没有。两种缝线在吻合口愈合方面没有显著差异。
ASC生物缝线对大鼠吻合口愈合没有影响。
