用于细胞移植的生物活性缝线的吸收性与拉伸性

Absorption and Tensility of Bioactive Sutures Prepared for Cell Transplantation.

作者信息

Horváthy Dénes B, Vácz Gabriella, Szabó Tamás, Renner Károly, Vajda Kinga, Sándor Balázs, Lacza Zsombor

机构信息

Institute of Human Physiology and Clinical Experimental Research, Semmelweis University, Tűzoltó 37-47, Budapest H-1094, Hungary.

Department of Orthopedics, Semmelweis University, Karolina 27, Budapest H-1113, Hungary.

出版信息

Materials (Basel). 2013 Feb 15;6(2):544-550. doi: 10.3390/ma6020544.

DOI:10.3390/ma6020544

PMID:28809324

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC5452094/

Abstract

Biodegradable scaffolds are widely used to transplant stem cells into various tissues. Recent studies showed that living stem cells can be attached to the surface of absorbable sutures . Soaking the absorbable material polyglactin in a cell culture medium and thereby creating a stem cell biofilm on its surface may initiate the absorption process even before implantation; therefore, the physicochemical properties of the suture may be compromised . We found that pre-incubation of sutures in cell culture media results in tensile strength reduction and faster suture absorption in a rat model of muscle injury. Shorter incubation times of up to 48 h do not influence absorption or tensility; therefore, it is advisable to limit incubation times to two days for polyglactin-based cell delivery protocols.

摘要

可生物降解支架被广泛用于将干细胞移植到各种组织中。最近的研究表明，活的干细胞可以附着在可吸收缝线的表面。将可吸收材料聚乙交酯丙交酯浸泡在细胞培养基中，从而在其表面形成干细胞生物膜，甚至在植入前就可能启动吸收过程；因此，缝线的物理化学性质可能会受到影响。我们发现，在细胞培养基中对缝线进行预孵育会导致大鼠肌肉损伤模型中的拉伸强度降低和缝线吸收加快。长达48小时的较短孵育时间不会影响吸收或拉伸性；因此，对于基于聚乙交酯的细胞递送方案，建议将孵育时间限制在两天。