Bölgen Nimet, Vargel Ibrahim, Korkusuz Petek, Güzel Elif, Plieva Fatima, Galaev Igor, Matiasson Bo, Pişkin Erhan
Chemical Engineering Department and Bioengineering Division, Hacettepe University, Beytepe, 06800 Ankara, Turkey.
J Biomed Mater Res A. 2009 Oct;91(1):60-8. doi: 10.1002/jbm.a.32193.
Biodegradable macroporous cryogels with highly open and interconnected pore structures were produced from dextran modified with oligo L-lactide bearing hydroxyethylmethacrylate (HEMA) end groups in moderately frozen solutions. Tissue responses to these novel scaffolds were evaluated in rats after dorsal subcutaneous implantation, iliac submuscular implantation, auricular implantation, or in calvarial defect model. In no case, either necrosis or foreign body reaction was observed during histological studies. The cryogel scaffolds integrated with the surrounding tissue and the formation of a new tissue were accompanied with significant ingrowth of connective tissue cells and new blood vessels into the cryogel. The tissue responses were significantly lower in auricular and calvarial implantations when compared with the subcutanous and the submuscular implantations. The degradation of the scaffold was slower in bone comparing to soft tissues. The biodegradable cryogels are highly biocompatible and combine extraordinary properties including having soft and elastic nature, open porous structure, and very rapid and controllable swelling. Therefore, the cryogels could be promising candidates for further clinical applications in tissue regeneration.
通过在适度冷冻的溶液中，用带有甲基丙烯酸羟乙酯（HEMA）端基的低聚L-丙交酯修饰葡聚糖，制备出具有高度开放且相互连通的孔结构的可生物降解大孔冷冻凝胶。在大鼠背部皮下植入、髂肌下植入、耳廓植入或颅骨缺损模型后，评估了组织对这些新型支架的反应。在组织学研究中，均未观察到坏死或异物反应。冷冻凝胶支架与周围组织整合，新组织的形成伴随着结缔组织细胞和新血管大量长入冷冻凝胶。与皮下和肌下植入相比，耳廓和颅骨植入时组织反应明显更低。与软组织相比，支架在骨中的降解更慢。这种可生物降解的冷冻凝胶具有高度生物相容性，兼具多种非凡特性，包括质地柔软且有弹性、开放的多孔结构以及非常快速且可控的溶胀性。因此，冷冻凝胶有望在组织再生的进一步临床应用中成为有潜力的候选材料。
