Department of Plastic Reconstructive and Aesthetic Surgery, Faculty of Medicine, Hacettepe University, Ankara, Turkey.
Department of Bioengineering, Faculty of Engineering, Hacettepe University, Ankara, Turkey.
Lasers Med Sci. 2022 Feb;37(1):595-606. doi: 10.1007/s10103-021-03308-y. Epub 2021 Apr 11.
Reconstruction of bone defects is still a significant challenge. The aim of this study was to evaluate the effect of application of photobiomodulation (PBM) to enhance in vivo bone regeneration and osteogenic differentiation potential of adipose-derived stem cells (ADSCs) encapsulated in methacrylated gelatin (GEL-MA) hydrogels. Thirty-six Sprague-Dawley rats were randomly separated into 3 experimental groups (n = 12 each). The groups were control/blank defect (I), GEL-MA hydrogel (II), and ADSC-loaded GEL-MA (GEL-MA+ADSC) hydrogel (III). Biparietal critical sized bone defects (6 mm in size) are created in each animal. Half of the animals from each group (n = 6 each) were randomly selected for PBM application using polychromatic light in the near infrared region, 600-1200 nm. PBM was administered from 10 cm distance cranially in 48 h interval. The calvaria were harvested at the 20th week, and macroscopic, microtomographic, and histologic evaluation were performed for further analysis. Microtomographic evaluation demonstrated the highest result for mineralized matrix formation (MMF) in group III. PBM receiving samples of group III showed mean MMF of 79.93±3.41%, whereas the non-PBM receiving samples revealed mean MMF of 60.62±6.34 % (p=0.002). In terms of histologic evaluation of bone defect repair, the higher scores were obtained in the groups II and III when compared to the control group (2.0 for both PBM receiving and non-receiving specimens; p<0.001). ADSC-loaded microwave-induced GEL-MA hydrogels and periodic application of photobiomodulation with polychromatic light appear to have beneficial effect on bone regeneration and can stimulate ADSCs for osteogenic differentiation.
骨缺损的重建仍然是一个重大挑战。本研究旨在评估应用光生物调节（PBM）来增强包封在甲基丙烯酰化明胶（GEL-MA）水凝胶中的脂肪来源干细胞（ADSCs）的体内骨再生和成骨分化潜力。36 只 Sprague-Dawley 大鼠被随机分为 3 个实验组（每组 n = 12）。组 I 为空白缺陷对照/空白缺陷（I），组 II 为 GEL-MA 水凝胶（II），组 III 为负载 ADSC 的 GEL-MA（GEL-MA+ADSC）水凝胶（III）。在每个动物中都创建了双额临界尺寸的骨缺损（大小为 6mm）。每组一半的动物（每组 n = 6）被随机选择接受近红外区域（600-1200nm）的多色光 PBM 应用。PBM 以 48 小时的间隔从 10cm 距离颅顶给药。颅骨在第 20 周收获，并进行宏观、微断层和组织学评估以进行进一步分析。微断层评估显示，组 III 的矿化基质形成（MMF）结果最高。接受 PBM 的组 III 的样本的平均 MMF 为 79.93±3.41%，而未接受 PBM 的样本的平均 MMF 为 60.62±6.34%（p=0.002）。在骨缺损修复的组织学评估方面，与对照组相比，组 II 和 III 的评分较高（接受和不接受 PBM 的样本均为 2.0；p<0.001）。负载 ADSC 的微波诱导 GEL-MA 水凝胶和周期性应用多色光光生物调节似乎对骨再生有有益的影响，并可以刺激 ADSC 进行成骨分化。
